Anti-CYP3A4 Polyclonal Antibody (HY264014)

Name: Anti-CYP3A4 Polyclonal Antibody
Brand: abinScience
SKU: HY264014
Price: 168 USD
Availability: InStock

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100ug

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개요
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참고 문헌
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SDS

개요

카탈로그 번호

HY264014

설명

Anti-CYP3A4 Polyclonal Antibody (HY264014) is a rabbit polyclonal antibody detecting CYP3A4 in ELISA, IHC, WB. Suitable for Human, Callithrix jacchus, and Macaca fascicularis.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

종 반응성

Human

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human CYP3A4 (Gly26-Ala503).

타겟

CYPIIIA3, Cytochrome P450 3A4, 1, 4-cineole 2-exo-monooxygenase, CYP3A4, Cytochrome P450 3A3, Cytochrome P450 NF-25, Cytochrome P450-PCN1, CYPIIIA4, 1, 8-cineole 2-exo-monooxygenase, Albendazole monooxygenase (sulfoxide-forming), Nifedipine oxidase, Quinine 3-monooxygenase, CYP3A3, Cholesterol 25-hydroxylase, Cytochrome P450 HLp, Albendazole sulfoxidase

정제

Purified by antigen affinity column.

등록번호

P08684

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Cytochrome P450 3A4 (CYP3A4) is a ~57 kDa protein. A cytochrome P450 monooxygenase involved in the metabolism of sterols, steroid hormones, retinoids and fatty acids. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds. Exhibits high catalytic activity for the formation of hydroxyestrogens from estrone (E1) and 17beta-estradiol (E2), namely 2-hydroxy E1 and E2, as well as D-ring hydroxylated E1 and E2 at the C-16 position. Plays a role in the metabolism of androgens, particularly in oxidative deactivation of testosterone.

1. Chen, H. et al. (2000) Drug metabolism and disposition: the biological fate of chemicals 28, 315-22. PMID: 10681376

2. Marill, J. et al. (2000) Molecular pharmacology 58, 1341-8. PMID: 11093772

3. Badawi, AF. et al. (2001) Metabolism: clinical and experimental 50, 1001-3. PMID: 11555828

4. Lee, AJ. et al. (2003) Endocrinology 144, 3382-98. PMID: 12865317

5. Lee, AJ. et al. (2003) Cancer research 63, 6532-6. PMID: 14559847

6. Krauser, JA. et al. (2004) European journal of biochemistry 271, 3962-9. PMID: 15373842

7. Choi, MH. et al. (2005) Drug metabolism and disposition: the biological fate of chemicals 33, 714-8. PMID: 15764715

8. Lucas, D. et al. (2010) Journal of lipid research 51, 1125-33. PMID: 19965576

9. Pratt-Hyatt, M. et al. (2010) Drug metabolism and disposition: the biological fate of chemicals 38, 2075-82. PMID: 20702771

10. Peng, CC. et al. (2011) Clinical pharmacology and therapeutics 89, 888-95. PMID: 21490593

참고사항

For research use only.

이미지

참고 문헌

데이터시트

Anti-CYP3A4 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-CYP3A4 Polyclonal Antibody (HY264014)

개요

이미지

참고 문헌

데이터시트

SDS