Anti-DDX58 Polyclonal Antibody (HF777014)

Name: Anti-DDX58 Polyclonal Antibody
Brand: abinScience
SKU: HF777014
Price: 168 USD
Availability: InStock

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개요
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참고 문헌
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SDS

개요

카탈로그 번호

HF777014

설명

Anti-DDX58 Polyclonal Antibody (HF777014) is a rabbit polyclonal antibody detecting DDX58 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

종 반응성

Human

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human DDX58 (Ile725-Lys925).

타겟

Probable ATP-dependent RNA helicase DDX58, Retinoic acid-inducible gene I protein, RIG-1, DEAD box protein 58, RLR-1, DDX58, Retinoic acid-inducible gene 1 protein, Antiviral innate immune response receptor RIG-I, RIG-I-like receptor 1, RIG-I

정제

Purified by antigen affinity column.

등록번호

O95786

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Antiviral innate immune response receptor RIG-I (DDX58) is a ~106 kDa protein. Innate immune receptor that senses cytoplasmic viral nucleic acids and activates a downstream signaling cascade leading to the production of type I interferons and pro-inflammatory cytokines. Forms a ribonucleoprotein complex with viral RNAs on which it homooligomerizes to form filaments. The homooligomerization allows the recruitment of RNF135 an E3 ubiquitin-protein ligase that activates and amplifies the RIG-I-mediated antiviral signaling in an RNA length-dependent manner through ubiquitination-dependent and -independent mechanisms. Upon activation, associates with mitochondria antiviral signaling protein (MAVS/IPS1) that activates the IKK-related kinases TBK1 and IKBKE which in turn phosphorylate the interferon regulatory factors IRF3 and IRF7, activating transcription of antiviral immunological genes including the IFN-alpha and IFN-beta interferons. Ligands include 5'-triphosphorylated ssRNAs and dsRNAs but also short dsRNAs (<1 kb in length).

1. Yoneyama, M. et al. (2004) Nature immunology 5, 730-7. PMID: 15208624

2. Sumpter, R. et al. (2005) Journal of virology 79, 2689-99. PMID: 15708988

3. Seth, RB. et al. (2005) Cell 122, 669-82. PMID: 16125763

4. Kawai, T. et al. (2005) Nature immunology 6, 981-8. PMID: 16127453

5. Xu, LG. et al. (2005) Molecular cell 19, 727-40. PMID: 16153868

6. Saito, T. et al. (2007) Proceedings of the National Academy of Sciences of the United States of America 104, 582-7. PMID: 17190814

7. Friedman, CS. et al. (2008) EMBO reports 9, 930-6. PMID: 18636086

8. Mukherjee, A. et al. (2009) The Journal of biological chemistry 284, 6486-94. PMID: 19122199

9. Bamming, D. et al. (2009) The Journal of biological chemistry 284, 9700-12. PMID: 19211564

10. Cui, J. et al. (2014) Cell research 24, 400-16. PMID: 24366338

참고사항

For research use only.

이미지

참고 문헌

데이터시트

Anti-DDX58 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-DDX58 Polyclonal Antibody (HF777014)

개요

이미지

참고 문헌

데이터시트

SDS