Anti-DGKE Polyclonal Antibody (HW773014)

Name: Anti-DGKE Polyclonal Antibody
Brand: abinScience
SKU: HW773014
Price: 168 USD
Availability: InStock

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개요
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참고 문헌
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개요

카탈로그 번호

HW773014

설명

Anti-DGKE Polyclonal Antibody (HW773014) is a rabbit polyclonal antibody detecting DGKE in ELISA, IHC, WB. Suitable for Human and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

종 반응성

Human, Mouse

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human DGKE (Leu243-Gly502).

타겟

DGKE, Diglyceride kinase epsilon, DAG kinase epsilon, DGK-epsilon, Diacylglycerol kinase epsilon, DAGK5

정제

Purified by antigen affinity column.

등록번호

P52429

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Diacylglycerol kinase epsilon (DGKE) is a ~63 kDa protein. Membrane-bound diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids. Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes. Also plays an important role in the biosynthesis of complex lipids. Displays specificity for diacylglycerol substrates with an arachidonoyl acyl chain at the sn-2 position, with the highest activity toward 1-octadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycerol the main diacylglycerol intermediate within the phosphatidylinositol turnover cycle. Can also phosphorylate diacylglycerol substrates with a linoleoyl acyl chain at the sn-2 position but much less efficiently.

1. Epand, RM. et al. (2004) Biochemistry 43, 14778-83. PMID: 15544348

2. Lung, M. et al. (2009) The Journal of biological chemistry 284, 31062-73. PMID: 19744926

3. Shulga, YV. et al. (2011) Journal of molecular biology 409, 101-12. PMID: 21477596

4. Shulga, YV. et al. (2011) FEBS letters 585, 4025-8. PMID: 22108654

5. Sato, M. et al. (2013) Pharmacology 92, 99-107. PMID: 23949095

6. Tang, W. et al. (1996) The Journal of biological chemistry 271, 10237-41. PMID: 8626589

참고사항

For research use only.

이미지

참고 문헌

데이터시트

Anti-DGKE Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-DGKE Polyclonal Antibody (HW773014)

개요

이미지

참고 문헌

데이터시트

SDS