Anti-DHFR Polyclonal Antibody (HF957014)

Name: Anti-DHFR Polyclonal Antibody
Brand: abinScience
SKU: HF957014
Price: 168 USD
Availability: InStock

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100ug

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개요
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참고 문헌
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개요

카탈로그 번호

HF957014

설명

Anti-DHFR Polyclonal Antibody (HF957014) is a rabbit polyclonal antibody detecting DHFR in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Pig.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

종 반응성

Human

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human DHFR (Met1-Asp187).

타겟

Dihydrofolate reductase, DHFR

정제

Purified by antigen affinity column.

등록번호

P00374

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Dihydrofolate reductase (DHFR) is a ~21 kDa protein. Catalyzes the reduction of 7,8-dihydrofolate (DHF) to 5,6,7,8-tetrahydrofolate in a NADPH-dependent manner. Key enzyme in folate metabolism. Contributes to the nuclear and mitochondrial de novo thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFR2.

1. Klon, AE. et al. (2002) Journal of molecular biology 320, 677-93. PMID: 12096917

2. Cody, V. et al. (2004) Acta crystallographica. Section D, Biological crystallography 60, 646-55. PMID: 15039552

3. Reynolds, RC. et al. (2007) Journal of medicinal chemistry 50, 3283-9. PMID: 17569517

4. Cody, V. et al. (2009) Biochemistry 48, 1702-11. PMID: 19196009

5. Volpato, JP. et al. (2009) The Journal of biological chemistry 284, 20079-89. PMID: 19478082

6. McEntee, G. et al. (2011) Proceedings of the National Academy of Sciences of the United States of America 108, 15157-62. PMID: 21876184

7. Gangjee, A. et al. (1998) Journal of medicinal chemistry 41, 3426-34. PMID: 9719595

8. Anderson, DD. et al. (2011) Proceedings of the National Academy of Sciences of the United States of America 108, 15163-8. PMID: 21876188

9. Anderson, DD. et al. (2012) The Journal of biological chemistry 287, 7051-62. PMID: 22235121

참고사항

For research use only.

이미지

참고 문헌

데이터시트

Anti-DHFR Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-DHFR Polyclonal Antibody (HF957014)

개요

이미지

참고 문헌

데이터시트

SDS