Anti-DNM2 Polyclonal Antibody (HW618014)

Name: Anti-DNM2 Polyclonal Antibody
Brand: abinScience
SKU: HW618014
Price: 96 USD
Availability: InStock

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개요
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참고 문헌
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SDS

개요

카탈로그 번호

HW618014

설명

Anti-DNM2 Polyclonal Antibody (HW618014) is a rabbit polyclonal antibody detecting DNM2 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

종 반응성

Human, Mouse, Rat

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human DNM2 (Met1-Gly230).

타겟

DNM2, Dynamin-2, DYN2

정제

Purified by antigen affinity column.

등록번호

P50570

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Dynamin-2 (DNM2) is a ~98 kDa protein. Catalyzes the hydrolysis of GTP and utilizes this energy to mediate vesicle scission at plasma membrane during endocytosis and filament remodeling at many actin structures during organization of the actin cytoskeleton. Plays an important role in vesicular trafficking processes, namely clathrin-mediated endocytosis (CME), exocytic and clathrin-coated vesicle from the trans-Golgi network, and PDGF stimulated macropinocytosis. During vesicular trafficking process, associates to the membrane, through lipid binding, and self-assembles into ring-like structure through oligomerization to form a helical polymer around the vesicle membrane and leading to vesicle scission. Plays a role in organization of the actin cytoskeleton by mediating arrangement of stress fibers and actin bundles in podocytes. During organization of the actin cytoskeleton, self-assembles into ring-like structure that directly bundles actin filaments to form typical membrane tubules decorated with dynamin spiral polymers.

1. Züchner, S. et al. (2005) Nature genetics 37, 289-94. PMID: 15731758

2. Mooren, OL. et al. (2009) The Journal of biological chemistry 284, 23995-4005. PMID: 19605363

3. Bitoun, M. et al. (2009) Human mutation 30, 1419-27. PMID: 19623537

4. Yeung, ML. et al. (2021) Cell 184, 2212-2228.e12. PMID: 33713620

5. Tassin, TC. et al. (2021) Frontiers in cellular neuroscience 15, 745940. PMID: 34744632

6. Fabrizi, GM. et al. (2007) Neurology 69, 291-5. PMID: 17636067

8. Søreng, K. et al. (2018) EMBO reports 19. PMID: 29437695

참고사항

For research use only.

이미지

Western blot

Western blot analysis was performed using anti-DNM2 polyclonal antibody at 1µg/ml on various samples.
Lane 1: Mouse brain lysate
Lane 2: Rat brain lysate

참고 문헌

데이터시트

Anti-DNM2 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-DNM2 Polyclonal Antibody (HW618014)

개요

이미지

참고 문헌

데이터시트

SDS