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Anti-E. coli groES Polyclonal Antibody (JN912014)

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개요
카탈로그 번호JN912014
설명
Anti-E. coli groES Polyclonal Antibody (JN912014) is a rabbit polyclonal antibody detecting groES. Suitable for strain K12.
종 반응성Escherichia coli
응용 분야ELISA, IHC, WB
숙주 종Rabbit
클론성Polyclonal
이소타입IgG
면역원 E. coli - derived recombinant E. coli groES (Met1-Ala97).
타겟 10 kDa chaperonin, Chaperonin-10, Co-chaperonin GroES, Cpn10, groES, groS, mopB
내독소 수준 Please contact with the lab for this information.
정제 Purified by antigen affinity column.
등록번호 P0A6F9
형태 Liquid
저장 완충액 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

재구성 Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
배송 In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
제품 사용 정보
응용 분야 희석
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
안정성 및 저장 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
배경

Co-chaperonin GroES is a ~10 kDa protein. Together with the chaperonin GroEL, plays an essential role in assisting protein folding. The GroEL-GroES system forms a nano-cage that allows encapsulation of the non-native substrate proteins and provides a physical environment optimized to promote and accelerate protein folding, probably by preventing aggregation and by entropically destabilizing folding intermediates. GroES binds to the apical surface of the GroEL ring, thereby capping the opening of the GroEL channel. Regulates the ATPase activity of GroEL.

1. Goloubinoff, P. et al. (1989) Nature 342, 884-9. PMID: 10532860
2. Tang, YC. et al. (2006) Cell 125, 903-14. PMID: 16751100
3. Martin, J. et al. (1991) Nature 352, 36-42. PMID: 1676490
4. Tang, YC. et al. (2008) The EMBO journal 27, 1458-68. PMID: 18418386
5. Apetri, AC. et al. (2008) Proceedings of the National Academy of Sciences of the United States of America 105, 17351-5. PMID: 18987317
6. Chakraborty, K. et al. (2010) Cell 142, 112-22. PMID: 20603018
7. Gupta, AJ. et al. (2014) Journal of molecular biology 426, 2739-54. PMID: 24816391
8. Kusukawa, N. et al. (1989) The EMBO journal 8, 3517-21. PMID: 2573517
9. Hemmingsen, SM. et al. (1988) Nature 333, 330-4. PMID: 2897629
참고사항 For research use only
이미지
참고 문헌
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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