Anti-EBV/HHV4 EBNA1/BKRF1 Polyclonal Antibody (VK686014)

Name: Anti-EBV/HHV4 EBNA1/BKRF1 Polyclonal Antibody
Brand: abinScience
SKU: VK686014
Price: 168 USD
Availability: InStock

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개요
이미지
참고 문헌
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SDS

개요

카탈로그 번호

VK686014

설명

Anti-EBV/HHV4 EBNA1/BKRF1 Polyclonal Antibody (VK686014) is a rabbit polyclonal antibody detecting EBNA1 in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

종 반응성

Epstein-Barr virus (HHV-4) (Human herpesvirus 4)

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant EBV/HHV4 EBNA1/BKRF1 (His468-Pro607).

타겟

Epstein-Barr nuclear antigen 1, EBNA-1, EBV nuclear antigen 1, EBNA1, BKRF1

정제

Purified by antigen affinity column.

등록번호

P03211

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Epstein-Barr nuclear antigen 1 (EBNA1/BKRF1) is a ~56 kDa protein. Responsible for the origin of replication (oriP) dependent replication and maintenance of viral episomes during latent infection. EBNA1 dimer interacts with the DS (dyad symmetry) element within the origin of replication oriP and with a host mitotic chromosome to initiate viral DNA replication during latency. EBNA1 binding to DS recruits the host origin recognition complex (ORC). Governs the faithful mitotic segregation of the viral episomes by binding both the FR (family of repeats) element within oriP and the host mitotic chromosomes. Forms a cell cycle-dependent tyrosine-dependent DNA cross-link and single-strand cleavage at oriP required for terminating replication and maintaining viral episomes.

1. Sears, J. et al. (2004) Journal of virology 78, 11487-505. PMID: 15479791

2. Rawlins, DR. et al. (1985) Cell 42, 859-68. PMID: 2996781

3. Hodin, TL. et al. (2013) Journal of virology 87, 13020-8. PMID: 24067969

4. Malecka, KA. et al. (2019) Journal of virology 93. PMID: 31142669

5. Summers, H. et al. (1996) Journal of virology 70, 1228-31. PMID: 8551585

6. Ritzi, M. et al. (2003) Journal of cell science 116, 3971-84. PMID: 12953058

7. Hung, SC. et al. (2001) Proceedings of the National Academy of Sciences of the United States of America 98, 1865-70. PMID: 11172042

참고사항

For research use only.

이미지

Western blot

Western blot analysis was performed using anti-EBNA1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant EBV/HHV4 EBNA1/BKRF1 (Catalog No: VK686012)

참고 문헌

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