Anti-Human EGFR Polyclonal Antibody (HF004024)

Name: Anti-Human EGFR Polyclonal Antibody
Brand: abinScience
SKU: HF004024
Price: 168 USD
Availability: InStock

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개요
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참고 문헌
데이터시트
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개요

카탈로그 번호

HF004024

설명

Anti-EGFR/ERBB1/HER1 Polyclonal Antibody (HF004024) is a rabbit polyclonal antibody detecting ERBB1 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

종 반응성

Human, Mouse

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human EGFR/ERBB1/HER1 (Leu25-Ser151).

타겟

EC:2.7.10.1, EGFR, ERBB, ERBB1, Epidermal growth factor receptor, HER1, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1

내독소 수준

Please contact with the lab for this information.

순도

>90% as determined by SDS-PAGE.

정제

Purified by antigen affinity column.

등록번호

P00533

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Epidermal growth factor receptor (EGFR/ERBB1) is a ~134 kDa protein. Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. EGFR is the therapeutic target of cetuximab (Erbitux) and panitumumab (Vectibix).

1. Arcaro, A. et al. (2000) Molecular and cellular biology 20, 3817-30. PMID: 10805725

2. Runkle, KB. et al. (2016) Molecular cell 62, 385-396. PMID: 27153536

3. Chen, WS. et al. (1989) Cell 59, 33-43. PMID: 2790960

4. Bu, J. et al. (2022) Acta biochimica et biophysica Sinica 54, 400-408. PMID: 35538033

5. Garrett, TP. et al. (2002) Cell 110, 763-73. PMID: 12297049

6. Kochupurakkal, BS. et al. (2005) The Journal of biological chemistry 280, 8503-12. PMID: 15611079

7. Kim, J. et al. (2007) Cancer research 67, 9229-37. PMID: 17909029

8. Lu, C. et al. (2010) Molecular and cellular biology 30, 5432-43. PMID: 20837704

참고사항

For research use only.

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