Anti-ERN1 Polyclonal Antibody (HF537014)

Name: Anti-ERN1 Polyclonal Antibody
Brand: abinScience
SKU: HF537014
Price: 168 USD
Availability: InStock

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개요
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개요

카탈로그 번호

HF537014

설명

Anti-ERN1 Polyclonal Antibody (HF537014) is a rabbit polyclonal antibody detecting ERN1 in ELISA, IHC, WB. Suitable for Human and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

종 반응성

Human, Mouse

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human ERN1 (Phe571-Leu977).

타겟

Endoplasmic reticulum-to-nucleus signaling 1, IRE1a, ERN1, Ire1-alpha, hIRE1p, Serine/threonine-protein kinase/endoribonuclease IRE1, Inositol-requiring protein 1, IRE1

정제

Purified by antigen affinity column.

등록번호

O75460

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Serine/threonine-protein kinase/endoribonuclease IRE1 (ERN1) is a ~109 kDa protein. Serine/threonine-protein kinase and endoribonuclease that acts as a key sensor for the endoplasmic reticulum unfolded protein response (UPR). In unstressed cells, the endoplasmic reticulum luminal domain is maintained in its inactive monomeric state by binding to the endoplasmic reticulum chaperone HSPA5/BiP. Accumulation of misfolded proteins in the endoplasmic reticulum causes release of HSPA5/BiP, allowing the luminal domain to homodimerize, promoting autophosphorylation of the kinase domain and subsequent activation of the endoribonuclease activity. The endoribonuclease activity is specific for XBP1 mRNA and excises 26 nucleotides from XBP1 mRNA. The resulting spliced transcript of XBP1 encodes a transcriptional activator protein that up-regulates expression of UPR target genes.

1. Iwawaki, T. et al. (2001) Nature cell biology 3, 158-64. PMID: 11175748

2. Yoshida, H. et al. (2001) Cell 107, 881-91. PMID: 11779464

3. Liu, CY. et al. (2003) The Journal of biological chemistry 278, 17680-7. PMID: 12637535

4. Lisbona, F. et al. (2009) Molecular cell 33, 679-91. PMID: 19328063

5. Ali, MM. et al. (2011) The EMBO journal 30, 894-905. PMID: 21317875

6. Liu, J. et al. (2017) Nature communications 8, 14186. PMID: 28128204

7. Chang, TK. et al. (2018) Molecular cell 71, 629-636.e5. PMID: 30118681

8. Kovaleva, V. et al. (2023) Cell reports 42, 112066. PMID: 36739529

9. Tirasophon, W. et al. (1998) Genes & development 12, 1812-24. PMID: 9637683

10. Eletto, D. et al. (2014) Molecular cell 53, 562-576. PMID: 24508390

참고사항

For research use only.

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참고 문헌

데이터시트

Anti-ERN1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-ERN1 Polyclonal Antibody (HF537014)

개요

이미지

참고 문헌

데이터시트

SDS