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| 카탈로그 번호 | HS927014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 종 반응성 | Human, Mouse | ||||||||
| 응용 분야 | ELISA, IHC, WB | ||||||||
| 숙주 종 | Rabbit | ||||||||
| 클론성 | Polyclonal | ||||||||
| 이소타입 | IgG | ||||||||
| 면역원 | E. coli - derived recombinant Human EZH2 (Arg608-Pro746). | ||||||||
| 타겟 | ENX-1, Enhancer of zeste homolog 2, EZH2, Lysine N-methyltransferase 6, KMT6, Histone-lysine N-methyltransferase EZH2 | ||||||||
| 정제 | Purified by antigen affinity column. | ||||||||
| 등록번호 | Q15910 | ||||||||
| 형태 | Liquid | ||||||||
| 저장 완충액 | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
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| 제품 사용 정보 |
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| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 배경 | Histone-lysine N-methyltransferase EZH2 is a ~85 kDa protein. Catalytic subunit of the PRC2/EED-EZH2 complex, a Polycomb group (PcG) complex that methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Displays a preference for substrates with less methylation, loses activity when progressively more methyl groups are incorporated into H3K27, H3K27me0 > H3K27me1 > H3K27me2. Compared to EZH1-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. 1. Bracken, AP. et al. (2003) The EMBO journal 22, 5323-35. PMID: 14532106 2. Cao, R. et al. (2004) Molecular cell 15, 57-67. PMID: 15225548 3. Pasini, D. et al. (2004) The EMBO journal 23, 4061-71. PMID: 15385962 4. Bracken, AP. et al. (2006) Genes & development 20, 1123-36. PMID: 16618801 5. Kim, DH. et al. (2006) Nature structural & molecular biology 13, 793-7. PMID: 16936726 6. Bracken, AP. et al. (2007) Genes & development 21, 525-30. PMID: 17344414 7. McCabe, MT. et al. (2012) Proceedings of the National Academy of Sciences of the United States of America 109, 2989-94. PMID: 22323599 8. Chu, CS. et al. (2014) Proceedings of the National Academy of Sciences of the United States of America 111, 1355-60. PMID: 24474760 9. Kim, JM. et al. (2015) Scientific reports 5, 16714. PMID: 26581166 10. Li, D. et al. (2018) Nature communications 9, 2829. PMID: 30026490 | ||||||||
| 참고사항 | For research use only. |
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