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Anti-GLI1 Polyclonal Antibody (HY371014)

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개요
카탈로그 번호HY371014
종 반응성Human
응용 분야ELISA, IHC, WB
숙주 종Rabbit
클론성Polyclonal
이소타입IgG
면역원 E. coli - derived recombinant Human GLI1 (Phe2-Glu234).
타겟 Oncogene GLI, GLI, Glioma-associated oncogene, GLI1, Zinc finger protein GLI1
정제 Purified by antigen affinity column.
등록번호 P08151
형태 Liquid
저장 완충액 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보
응용 분야 희석
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
안정성 및 저장 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
배경

Zinc finger protein GLI1 is a ~117 kDa protein. Acts as a transcriptional activator. Binds to the DNA consensus sequence 5'-GACCACCCA-3'. Regulates the transcription of specific genes during normal development. Plays a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling.

1. Murone, M. et al. (2000) Nature cell biology 2, 310-2. PMID: 10806483
2. Lo, HW. et al. (2009) Cancer research 69, 6790-8. PMID: 19706761
3. Maloverjan, A. et al. (2010) Experimental cell research 316, 627-37. PMID: 19878745
4. Gilder, AS. et al. (2013) Biochemical and biophysical research communications 440, 431-6. PMID: 24076122
5. Zhang, Y. et al. (2013) Nature communications 4, 2608. PMID: 24217340
6. Cherry, AL. et al. (2013) Acta crystallographica. Section D, Biological crystallography 69, 2563-79. PMID: 24311597
7. Kinzler, KW. et al. (1990) Molecular and cellular biology 10, 634-42. PMID: 2105456
8. Pavletich, NP. et al. (1993) Science (New York, N.Y.) 261, 1701-7. PMID: 8378770
참고사항 For research use only.
이미지
참고 문헌
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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