Anti-HAdV-5 E1B-55K Polyclonal Antibody (VK466074)

Name: Anti-HAdV-5 E1B-55K Polyclonal Antibody
Brand: abinScience
SKU: VK466074
Price: 168 USD
Availability: InStock

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사양:

50ug
100ug

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개요
이미지
참고 문헌
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SDS

개요

카탈로그 번호

VK466074

설명

Anti-HAdV-5 E1B-55K/E1B-495R Polyclonal Antibody (VK466074) is a rabbit polyclonal antibody detecting E1B-495R in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

종 반응성

Human adenovirus C serotype 5 (HAdV-5) (Human adenovirus 5), Human adenovirus C serotype 2 (HAdV-2)

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant HAdV-5 E1B-55K/E1B-495R (Met1-Gln79).

타겟

E1B 55 kDa protein, E1B-55K, E1B protein, large T-antigen, E1B-495R

내독소 수준

Please contact with the lab for this information.

정제

Purified by antigen affinity column.

등록번호

P03244, P03243

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000,IHC:1:50-1:500,WB:1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

E1B 55 kDa protein (E1B-55K/E1B-495R) is a ~54 kDa protein. Plays a major role to prevent cellular inhibition of viral genome replication. Assembles an SCF-like E3 ubiquitin ligase complex based on the cellular proteins ELOB, ELOC, CUL5 and RBX1, in cooperation with viral E4orf6. This viral RING-type ligase ubiquitinates cellular substrates and targets them to proteasomal degradation: TP53/p53, LIG4, MRE11-RAD50-NBS1 (MRN) complex, ITGA3, DAXX and BLM. E1B-55K probably acts as the substrate-specific adapter of the SCF-like E3 ubiquitin ligase complex. Degradation of host TP53/p53 activity is essential for preventing E1A-induced TP53 accumulation that would otherwise lead to cell apoptosis and growth arrest.

참고사항

For research use only

이미지

Western blot

Western blot analysis was performed using anti-E1BB5-ADE05 polyclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant HAdV-5 E1B-55K/E1B-495R (Catalog No: VK466072)

참고 문헌

데이터시트

Anti-HAdV-5 E1B-55K Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-HAdV-5 E1B-55K Polyclonal Antibody (VK466074)

개요

이미지

참고 문헌

데이터시트

SDS

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