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Anti-HEV Papain-like Cysteine Protease/PCP Polyclonal Antibody (VK620044)

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개요
카탈로그 번호VK620044
설명
Anti-Hepatitis E virus/HEV Papain-like Cysteine Protease/PCP Polyclonal Antibody (VK620044) is a rabbit polyclonal antibody detecting Putative papain-like cysteine protease. Suitable for Hepatitis E virus genotype 2.
종 반응성Hepatitis E virus (HEV)
응용 분야ELISA, IHC, WB
숙주 종Rabbit
클론성Polyclonal
이소타입IgG
면역원 E. coli - derived recombinant Hepatitis E virus/HEV Papain-like Cysteine Protease/PCP (Thr509-Ala691).
타겟 EC:2.1.1.-, EC:2.7.7.-, EC:2.7.7.48, EC:3.4.-.-, EC:3.6.4.-, Methyltransferase, NTPase/helicase, Non-structural polyprotein pORF1, PCP, Putative papain-like cysteine protease, Putative protease, RNA-directed RNA polymerase, RdRp
내독소 수준 Please contact with the lab for this information.
정제 Purified by antigen affinity column.
등록번호 Q03495
형태 Liquid
저장 완충액 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보
응용 분야 희석
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
배경

Non-structural polyprotein pORF1 (Papain-like Cysteine Protease/PCP) is a ~185 kDa protein. Methyltransferase: Displays a capping enzyme activity. This function is necessary since all viral RNAs are synthesized in the cytoplasm, and host capping enzymes are restricted to the nucleus. The enzymatic reaction involves a covalent link between 7-methyl-GMP and the methyltransferase, whereas eukaryotic capping enzymes form a covalent complex only with GMP. Methyltransferase catalyzes transfer of a methyl group from S-adenosylmethionine to GTP and GDP to yield m(7)GTP or m(7)GDP. GDP is a better substrate than GTP.

참고사항 For research use only
이미지
참고 문헌
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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