Anti-Histone H3 Polyclonal Antibody (HV603014)

Name: Anti-Histone H3 Polyclonal Antibody
Brand: abinScience
SKU: HV603014
Price: 96 USD
Availability: InStock

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개요
이미지
참고 문헌
데이터시트
SDS

개요

카탈로그 번호

HV603014

설명

Anti-Histone H3 Polyclonal Antibody (HV603014) is a rabbit polyclonal antibody detecting Histone H3/o in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Acropora formosa, and Aspergillus niger.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

종 반응성

Human, Mouse, Rat

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human H3C15 (Met1-Ala136).

타겟

HIST2H3A, H3C15, Histone H3/o, H3-clustered histone 14, H3-clustered histone 15, H3-clustered histone 13, H3F2, H3FM, Histone H3/m, HIST2H3D, HIST2H3C, Histone H3.2

내독소 수준

Please contact with the lab for this information.

정제

Purified by antigen affinity column.

등록번호

Q71DI3

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

재구성

Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.

제품 사용 정보

응용 분야	희석
ELISA	1:2000-1:20000
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Histone H3.2 (H3C15) is a ~15 kDa protein. Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

참고사항

For research use only.

이미지

Western blot

Western blot analysis was performed using anti-H3C15 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Hela cell lysate
Lane 2: HepG2 cell lysate
Lane 3: MCF-7 cell lysate
Lane 4: NIH3T3 cell lysate
Lane 5: A549 cell lysate
Lane 6: K562 cell lysate
Lane 7: Mouse spleen lysate

참고 문헌

데이터시트

Anti-Histone H3 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-Histone H3 Polyclonal Antibody (HV603014)

개요

이미지

참고 문헌

데이터시트

SDS

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