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Anti-Honeybee Api m 4/Melittin Polyclonal Antibody (ZA251014)

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개요
카탈로그 번호ZA251014
설명
Anti-Honeybee Api m 4/Melittin Polyclonal Antibody (ZA251014) is a rabbit polyclonal antibody detecting Melittin in ELISA, IHC, WB.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
종 반응성Apis mellifera (Honeybee)
응용 분야ELISA, IHC, WB
숙주 종Rabbit
클론성Polyclonal
이소타입IgG
면역원 E. coli - derived recombinant Honeybee Api m 4/Melittin (Gly44-Gln69).
타겟 Melittin, MEL, MLT, Allergen Api m 3, Allergen Api m III, Api m 4, MELT
정제 Purified by antigen affinity column.
등록번호 P01501
형태 Liquid
저장 완충액 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보
응용 분야 희석
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
안정성 및 저장 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
배경

Melittin (Api m 4) is a ~7 kDa protein. Melittin: Main toxin of bee venom with strong antimicrobial activity and hemolytic activity. It has enhancing effects on bee venom phospholipase A2 activity. This amphipathic toxin binds to negatively charged membrane surface and forms pore by inserting into lipid bilayers inducing the leakage of ions and molecules and the enhancement of permeability that ultimately leads to cell lysis. It acts as a voltage-gated pore with higher selectivity for anions over cations. The ion conductance has been shown to be voltage-dependent.

1. Park, D. et al. (2014) Peptides 53, 185-93. PMID: 24512991
2. Tosteson, MT. et al. (1985) The Journal of membrane biology 87, 35-44. PMID: 4057243
3. Schröder, E. et al. (1971) Experientia 27, 764-5. PMID: 5139482
4. Sessa, G. et al. (1969) The Journal of biological chemistry 244, 3575-82. PMID: 5794226
5. Mollay, C. et al. (1974) FEBS letters 46, 141-4. PMID: 4371280
6. Batenburg, AM. et al. (1987) FEBS letters 223, 148-54. PMID: 3666135
7. Hider, RC. et al. (1983) Biochimica et biophysica acta 728, 206-14. PMID: 6830776
8. Tosteson, MT. et al. (1981) Biophysical journal 36, 109-16. PMID: 6269667
9. Kempf, C. et al. (1982) The Journal of biological chemistry 257, 2469-76. PMID: 7061434
참고사항 For research use only.
이미지
참고 문헌
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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