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Anti-HPeV-1 VP1 Polyclonal Antibody (VK085034)

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개요
카탈로그 번호VK085034
설명
Anti-HPeV-1 VP1 Polyclonal Antibody (VK085034) is a rabbit polyclonal antibody detecting Capsid protein VP1 in ELISA, IHC, WB.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
종 반응성Human parechovirus 1 (HPeV-1)
응용 분야ELISA, IHC, WB
숙주 종Rabbit
클론성Polyclonal
이소타입IgG
면역원 E. coli - derived recombinant HPeV-1 Genome polyprotein (Thr608-Thr758).
타겟 Genome polyprotein, Capsid protein VP0, P1AB, Virion protein 0, Capsid protein VP3, P1C, Virion protein 3, Capsid protein VP1, P1D, Virion protein 1, Protein 2A H-NC, P2A, Protein 2A, Protein 2B, P2B, Protein 2C, P2C, 3.6.1.15, Protein 3A, P3A, Viral protein genome-linked, VPg, Protein 3B, P3B, Protease 3C, P3C, 3.4.22.28, Picornain 3C, RNA-directed RNA polymerase 3D-POL, P3D-POL, 2.7.7.48
내독소 수준 Please contact with the lab for this information.
순도 >95% as determined by SDS-PAGE.
정제 Purified by antigen affinity column.
등록번호 Q66578
형태 Liquid
저장 완충액 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

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제품 사용 정보
응용 분야 희석
ELISA 1:5000-1:20000,IHC:1:50-1:500,WB:1:500-1:2000
안정성 및 저장 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
배경

Genome polyprotein (VP1) is a ~245 kDa protein. Forms an icosahedral capsid of pseudo T=3 symmetry together with capsid proteins VP1 and VP3. The capsid is 300 Angstroms in diameter, composed of 60 copies of each capsid protein and enclosing the viral positive strand RNA genome. Capsid proteins interact with host alpha-V/beta-3 integrin heterodimer to provide virion attachment target cell. This attachment induces virion internalization predominantly through clathrin-mediated endocytosis. Binds packaging signals present in the viral RNA.

1. Joki-Korpela, P. et al. (2001) Journal of virology 75, 1958-67. PMID: 11160695
2. Shakeel, S. et al. (2017) Nature communications 8, 5. PMID: 28232749
참고사항 For research use only
이미지
참고 문헌
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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