Anti-HSPA1A Polyclonal Antibody (HY029014)

Name: Anti-HSPA1A Polyclonal Antibody
Brand: abinScience
SKU: HY029014
Price: 168 USD
Availability: InStock

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개요
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참고 문헌
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SDS

개요

카탈로그 번호

HY029014

설명

Anti-HSPA1A Polyclonal Antibody (HY029014) is a rabbit polyclonal antibody detecting HSPA1A in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Bos mutus grunniens.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

종 반응성

Human, Mouse, Rat

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human HSPA1A (Met1-Asp641).

타겟

HSP70.1, HSP70-1, HSPA1, HSPA1A, Heat shock 70 kDa protein 1A, Heat shock 70 kDa protein 1, HSP72, HSX70

정제

Purified by antigen affinity column.

등록번호

P0DMV8

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Heat shock 70 kDa protein 1A (HSPA1A) is a ~70 kDa protein. Molecular chaperone implicated in a wide variety of cellular processes, including protection of the proteome from stress, folding and transport of newly synthesized polypeptides, activation of proteolysis of misfolded proteins and the formation and dissociation of protein complexes. Plays a pivotal role in the protein quality control system, ensuring the correct folding of proteins, the re-folding of misfolded proteins and controlling the targeting of proteins for subsequent degradation. This is achieved through cycles of ATP binding, ATP hydrolysis and ADP release, mediated by co-chaperones. The co-chaperones have been shown to not only regulate different steps of the ATPase cycle, but they also have an individual specificity such that one co-chaperone may promote folding of a substrate while another may promote degradation. The affinity for polypeptides is regulated by its nucleotide bound state.

1. Mayer, MP. (2013) Trends in biochemical sciences 38, 507-14. PMID: 24012426

2. Rauch, JN. et al. (2014) The Journal of biological chemistry 289, 1402-14. PMID: 24318877

3. Radons, J. (2016) Cell stress & chaperones 21, 379-404. PMID: 26865365

4. Seo, JH. et al. (2016) Nature communications 7, 12882. PMID: 27708256

5. Fang, CT. et al. (2016) Cellular and molecular life sciences : CMLS 73, 3949-60. PMID: 27137183

6. Shang, Y. et al. (2014) Biochemical and biophysical research communications 446, 387-92. PMID: 24613385

7. Chen, Z. et al. (2013) Immunity 39, 272-85. PMID: 23973223

8. Shi, Y. et al. (1998) Genes & development 12, 654-66. PMID: 9499401

9. Wang, WF. et al. (2017) Nature communications 8, 363. PMID: 28842558

참고사항

For research use only.

이미지

참고 문헌

데이터시트

Anti-HSPA1A Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-HSPA1A Polyclonal Antibody (HY029014)

개요

이미지

참고 문헌

데이터시트

SDS

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