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| 카탈로그 번호 | HT428323 |
|---|---|
| 종 반응성 | Human |
| 응용 분야 | FCM |
| 숙주 종 | Mouse |
| 클론성 | Monoclonal |
| 이소타입 | VHH-mFc |
| 클론 ID | SAA1511 |
| 접합 | PE |
| 타겟 | ADAM 12, MLTN, Meltrin-alpha, Disintegrin and metalloproteinase domain-containing protein 12, ADAM12 |
| 내독소 수준 | Please contact with the lab for this information. |
| 순도 | >95% as determined by SDS-PAGE. |
| 관련 제품 | VHH-mFc Isotype Control antibody (cAb-Lys2), PE |
| 정제 | Protein A/G purified from cell culture supernatant. |
| 등록번호 | O43184 |
| RRID | Anti-Human ADAM12 Antibody (SAA1511), PE (abinScience Cat# HT428323, RRID:AB_3729856) |
| 형태 | Liquid |
| 저장 완충액 | 0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
| 재구성 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 안정성 및 저장 | Store at 4°C for 12 months. Protect from light. Do not freeze. |
| 배경 | Disintegrin and metalloproteinase domain-containing protein 12 (ADAM12) is a ~99 kDa protein. Involved in skeletal muscle regeneration, specifically at the onset of cell fusion. Also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors. |
| 참고사항 | For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only. |
| Isotype Control | Mouse IgG1, kappa Recombinant Isotype Control Antibody (MOPC-21), PE (MV080227) |
Flow-cytometry using PE anti-human ADAM12 antibody. U87 cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human ADAM12 monoclonal antibody (Catalog HT428323, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
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