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Anti-Human AK1/Adenylate kinase isoenzyme 1 Polyclonal Antibody (HF865014)

Anti-Human AK1/Adenylate kinase isoenzyme 1 Polyclonal Antibody
Anti-Human AK1/Adenylate kinase isoenzyme 1 Polyclonal Antibody
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개요
카탈로그 번호HF865014
설명
Anti-Human AK1/Adenylate kinase isoenzyme 1 Polyclonal Antibody (HF865014) is a rabbit polyclonal antibody detecting AK1/Adenylate kinase isoenzyme 1 in ELISA, IHC, WB. Suitable for Rattus norvegicus, Mus musculus, Human, Sus scrofa, and Bovine.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
종 반응성Human
응용 분야ELISA, IHC, WB
숙주 종Rabbit
클론성Polyclonal
이소타입IgG
면역원 E. coli - derived recombinant Human AK1/Adenylate kinase isoenzyme 1 (Met1-Lys194).
타겟 AK1/Adenylate kinase isoenzyme 1
정제 Purified by antigen affinity column.
등록번호 P00568
형태 Liquid
저장 완충액 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보
응용 분야 희석
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
안정성 및 저장 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
대체 이름Adenylate kinase isoenzyme 1, AK 1, 2.7.4.3, 2.7.4.6, ATP-AMP transphosphorylase 1, ATP:AMP phosphotransferase, Adenylate monophosphate kinase, Myokinase, AK1
배경

Adenylate kinase isoenzyme 1 (AK1) is a ~21 kDa protein. Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Also displays broad nucleoside diphosphate kinase activity. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism. Also catalyzes at a very low rate the synthesis of thiamine triphosphate (ThTP) from thiamine diphosphate (ThDP) and ADP.

1. Panayiotou, C. et al. (2011) The Biochemical journal 433, 527-34. PMID: 21080915
2. Amiri, M. et al. (2013) The international journal of biochemistry & cell biology 45, 925-31. PMID: 23416111
3. Matsuura, S. et al. (1989) The Journal of biological chemistry 264, 10148-55. PMID: 2542324
참고사항 For research use only.
이미지
  • Anti-Human AK1/Adenylate kinase isoenzyme 1 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-AK1 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Mouse heart lysate
    Lane 2: Mouse skeletal muscle lysate
    Lane 3: Rat heart lysate
    Lane 4: Rat skeletal muscle lysate

참고 문헌
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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