Anti-Human AKR1C3 Polyclonal Antibody (HW642014)

Name: Anti-Human AKR1C3 Polyclonal Antibody
Brand: abinScience
SKU: HW642014
Price: 168 USD
Availability: InStock

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100ug

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개요
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참고 문헌
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SDS

개요

카탈로그 번호

HW642014

설명

Anti-Human AKR1C3 Polyclonal Antibody (HW642014) is a rabbit polyclonal antibody detecting AKR1C3 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

종 반응성

Human

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human AKR1C3 (Met1-Tyr323).

타겟

3-alpha-hydroxysteroid dehydrogenase type 2, Aldo-keto reductase family 1 member C3, Testosterone 17-beta-dehydrogenase 5, 3-alpha-HSD type II, brain, DD3, DD-3, PGFS, Prostaglandin F synthase, 17-beta-HSD 5, Chlordecone reductase homolog HAKRb, Dihydrodiol dehydrogenase type I, Dihydrodiol dehydrogenase 3, HSD17B5, 17-beta-hydroxysteroid dehydrogenase type 5, HA1753, KIAA0119, DDH1, 3-alpha-HSD type 2, AKR1C3

정제

Purified by antigen affinity column.

등록번호

P42330

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Aldo-keto reductase family 1 member C3 (AKR1C3) is a ~36 kDa protein. Cytosolic aldo-keto reductase that catalyzes NADPH-dependent reduction of ketosteroids to hydroxysteroids. Displays broad substrate specificity with distinct positional and stereochemistry, primarily generating 17beta-hydroxysteroids, but also 3alpha- and 20alpha-hydroxysteroids. Produces potent androgens via classical and 'backdoor'/alternative pathways. In the classical androgen metabolic pathway (biosynthesis of 5alpha-dihydrotestosterone (5alpha-DHT) via testosterone), catalyzes the reduction of delta4-androstenedione to form testosterone. In the 'backdoor' androgen metabolic pathway (biosynthesis of 5alpha-dihydrotestosterone (5alpha-DHT) via pregnanes), reduces androsterone to 5alpha-androstane-3alpha,17beta-diol preceding 5alpha-DHT secretion.

1. Penning, TM. et al. (2000) The Biochemical journal 351, 67-77. PMID: 10998348

2. Penning, TM. et al. (2001) Molecular and cellular endocrinology 171, 137-49. PMID: 11165022

3. Byrns, MC. et al. (2010) The Journal of steroid biochemistry and molecular biology 118, 177-87. PMID: 20036328

4. Lin, HK. et al. (1997) Molecular endocrinology (Baltimore, Md.) 11, 1971-84. PMID: 9415401

5. Dufort, I. et al. (1999) Endocrinology 140, 568-74. PMID: 9927279

7. Steckelbroeck, S. et al. (2004) The Journal of biological chemistry 279, 10784-95. PMID: 14672942

8. Khanna, M. et al. (1995) The Journal of biological chemistry 270, 20162-8. PMID: 7650035

9. Endo, S. et al. (2020) The Journal of steroid biochemistry and molecular biology 199, 105586. PMID: 31926269

참고사항

For research use only.

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참고 문헌

데이터시트

Anti-Human AKR1C3 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-Human AKR1C3 Polyclonal Antibody (HW642014)

개요

이미지

참고 문헌

데이터시트

SDS