Anti-Human ALKBH2 Polyclonal Antibody (HD328014)

Name: Anti-Human ALKBH2 Polyclonal Antibody
Brand: abinScience
SKU: HD328014
Price: 168 USD
Availability: InStock

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50ug
100ug

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개요
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참고 문헌
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SDS

개요

카탈로그 번호

HD328014

설명

Anti-Human ALKBH2 Polyclonal Antibody (HD328014) is a rabbit polyclonal antibody detecting ALKBH2. Suitable for Human.

종 반응성

Human

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human ALKBH2 (Ser56-Lys261).

타겟

ABH2, ALKBH2, Alkylated DNA repair protein alkB homolog 2, Alpha-ketoglutarate-dependent dioxygenase alkB homolog 2, DNA oxidative demethylase ALKBH2, EC:1.14.11.33, Oxy DC1

내독소 수준

Please contact with the lab for this information.

정제

Purified by antigen affinity column.

등록번호

Q6NS38

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

배경

DNA oxidative demethylase ALKBH2 is a ~29 kDa protein. Dioxygenase that repairs alkylated nucleic acid bases by direct reversal oxidative dealkylation. Can process both double-stranded (ds) and single-stranded (ss) DNA substrates, with a strong preference for dsDNA. Uses molecular oxygen, 2-oxoglutarate and iron as cofactors to oxidize the alkyl groups that are subsequently released as aldehydes, regenerating the undamaged bases. Probes the base pair stability, locates a weakened base pair and flips the damaged base to accommodate the lesion in its active site for efficient catalysis. Repairs monoalkylated bases, specifically N1-methyladenine and N3-methylcytosine, as well as higher order alkyl adducts such as bases modified with exocyclic bridged adducts known as etheno adducts including 1,N6-ethenoadenine, 3,N4-ethenocytosine and 1,N2-ethenoguanine.

1. Duncan, T. et al. (2002) Proceedings of the National Academy of Sciences of the United States of America 99, 16660-5. PMID: 12486230

2. Aas, PA. et al. (2003) Nature 421, 859-63. PMID: 12594517

3. Lee, DH. et al. (2005) The Journal of biological chemistry 280, 39448-59. PMID: 16174769

4. Chen, B. et al. (2010) Molecular bioSystems 6, 2143-9. PMID: 20714506

5. Li, P. et al. (2013) Cell reports 4, 817-29. PMID: 23972994

6. Zdżalik, D. et al. (2015) DNA repair 30, 1-10. PMID: 25797601

7. Yang, CG. et al. (2008) Nature 452, 961-5. PMID: 18432238

8. Yi, C. et al. (2012) Nature structural & molecular biology 19, 671-6. PMID: 22659876

참고사항

For research use only

이미지

참고 문헌

데이터시트

Anti-Human ALKBH2 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-Human ALKBH2 Polyclonal Antibody (HD328014)

개요

이미지

참고 문헌

데이터시트

SDS