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| 카탈로그 번호 | HA094023 | ||||
|---|---|---|---|---|---|
| 종 반응성 | Human | ||||
| 응용 분야 | ELISA, IF, IP | ||||
| 숙주 종 | Mouse | ||||
| 클론성 | Monoclonal | ||||
| 이소타입 | IgG2a | ||||
| 클론 ID | SAA2126 | ||||
| 타겟 | ANKZF1, ZNF744, Ankyrin repeat and zinc finger domain containing 1, Ankyrin repeat and zinc finger domain-containing protein 1, Zinc finger protein 744 | ||||
| 내독소 수준 | Please contact with the lab for this information. | ||||
| 순도 | >95% as determined by SDS-PAGE. | ||||
| 정제 | Protein A/G purified from cell culture supernatant. | ||||
| 등록번호 | Q9H8Y5 | ||||
| RRID | Anti-Human ANKZF1 Antibody (SAA2126) (abinScience Cat# HA094023, RRID:AB_3729478) |
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| 형태 | Liquid | ||||
| 저장 완충액 | 0.01M PBS pH 7.4 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
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| 제품 사용 정보 |
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| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. | ||||
| 배경 | tRNA endonuclease ANKZF1 is a ~80 kDa protein. Endonuclease that cleaves polypeptidyl-tRNAs downstream of the ribosome-associated quality control (RQC) pathway to release incompletely synthesized polypeptides for degradation. The RQC pathway disassembles aberrantly stalled translation complexes to recycle or degrade the constituent parts. ANKZF1 acts downstream disassembly of stalled ribosomes and specifically cleaves off the terminal 3'-CCA nucleotides universal to all tRNAs from polypeptidyl-tRNAs, releasing (1) ubiquitinated polypeptides from 60S ribosomal subunit for degradation and (2) cleaved tRNAs. ANKZF1-cleaved tRNAs are then repaired and recycled by ELAC1 and TRNT1. Also plays a role in the cellular response to hydrogen peroxide and in the maintenance of mitochondrial integrity under conditions of cellular stress. 1. Verma, R. et al. (2018) Nature 557, 446-451. PMID: 29632312 2. Kuroha, K. et al. (2018) Molecular cell 72, 286-302.e8. PMID: 30244831 3. Yip, MCJ. et al. (2019) Nature structural & molecular biology 26, 343-349. PMID: 31011209 4. Yip, MCJ. et al. (2020) Cell reports 30, 2106-2114.e5. PMID: 32075755 5. van Haaften-Visser, DY. et al. (2017) The Journal of biological chemistry 292, 7904-7920. PMID: 28302725 | ||||
| 참고사항 | For research use only. |
SDS-PAGE for Anti-Human ANKZF1 Antibody (SAA2126)
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