Anti-Human ATG4A Polyclonal Antibody (HP478014)

Name: Anti-Human ATG4A Polyclonal Antibody
Brand: abinScience
SKU: HP478014
Price: 168 USD
Availability: InStock

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개요
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참고 문헌
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SDS

개요

카탈로그 번호

HP478014

설명

Anti-Human ATG4A Polyclonal Antibody (HP478014) is a rabbit polyclonal antibody detecting ATG4A in ELISA, IHC, WB. Suitable for Human, Mouse, Bovine, and Pongo abelii.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

종 반응성

Human, Mouse

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human ATG4A (Met1-Val398).

타겟

hAPG4A, Cysteine protease ATG4A, ATG4A, AUTL2, Autophagin-2, APG4A, AUT-like 2 cysteine endopeptidase, Autophagy-related protein 4 homolog A, Autophagy-related cysteine endopeptidase 2

정제

Purified by antigen affinity column.

등록번호

Q8WYN0

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Cysteine protease ATG4A is a ~45 kDa protein. Cysteine protease that plays a key role in autophagy by mediating both proteolytic activation and delipidation of ATG8 family proteins. The protease activity is required for proteolytic activation of ATG8 family proteins: cleaves the C-terminal amino acid of ATG8 proteins to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP. Protease activity is also required to counteract formation of high-molecular weight conjugates of ATG8 proteins (ATG8ylation): acts as a deubiquitinating-like enzyme that removes ATG8 conjugated to other proteins, such as ATG3.

1. Scherz-Shouval, R. et al. (2003) The Journal of biological chemistry 278, 14053-8. PMID: 12473658

2. Kabeya, Y. et al. (2004) Journal of cell science 117, 2805-12. PMID: 15169837

3. Scherz-Shouval, R. et al. (2007) The EMBO journal 26, 1749-60. PMID: 17347651

4. Li, M. et al. (2011) The Journal of biological chemistry 286, 7327-38. PMID: 21177865

5. Shu, CW. et al. (2011) Journal of biomolecular screening 16, 174-82. PMID: 21245471

6. Li, M. et al. (2012) Autophagy 8, 401-12. PMID: 22302004

7. Nguyen, N. et al. (2020) The Journal of biological chemistry 295, 13584-13600. PMID: 32732290

9. Nguyen, TN. et al. (2021) Molecular cell 81, 2013-2030.e9. PMID: 33773106

10. Kauffman, KJ. et al. (2018) Autophagy 14, 992-1010. PMID: 29458288

참고사항

For research use only.

이미지

참고 문헌

데이터시트

Anti-Human ATG4A Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-Human ATG4A Polyclonal Antibody (HP478014)

개요

이미지

참고 문헌

데이터시트

SDS