Anti-Human ATG4D Polyclonal Antibody (HC229014)

Name: Anti-Human ATG4D Polyclonal Antibody
Brand: abinScience
SKU: HC229014
Price: 168 USD
Availability: InStock

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100ug

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개요
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참고 문헌
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SDS

개요

카탈로그 번호

HC229014

설명

Anti-Human ATG4D Polyclonal Antibody (HC229014) is a rabbit polyclonal antibody detecting ATG4D. Suitable for Human.

종 반응성

Human

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human ATG4D (Ser92-Ser420).

타겟

APG4D, ATG4D, AUT-like 4 cysteine endopeptidase, AUTL4, Autophagin-4, Autophagy-related cysteine endopeptidase 4, Autophagy-related protein 4 homolog D, Cysteine protease ATG4D, Cysteine protease ATG4D, mitochondrial, EC:3.4.22.-, HsAPG4D

내독소 수준

Please contact with the lab for this information.

정제

Purified by antigen affinity column.

등록번호

Q86TL0

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

배경

Cysteine protease ATG4D is a ~52 kDa protein. Cysteine protease that plays a key role in autophagy by mediating both proteolytic activation and delipidation of ATG8 family proteins. The protease activity is required for proteolytic activation of ATG8 family proteins: cleaves the C-terminal amino acid of ATG8 proteins MAP1LC3 and GABARAPL2, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. In addition to the protease activity, also mediates delipidation of ATG8 family proteins. Catalyzes delipidation of PE-conjugated forms of ATG8 proteins during macroautophagy.

1. Li, M. et al. (2011) The Journal of biological chemistry 286, 7327-38. PMID: 21177865

2. Kauffman, KJ. et al. (2018) Autophagy 14, 992-1010. PMID: 29458288

3. Agrotis, A. et al. (2019) Autophagy 15, 976-997. PMID: 30661429

4. Durgan, J. et al. (2021) Molecular cell 81, 2031-2040.e8. PMID: 33909989

5. Nguyen, TN. et al. (2021) Molecular cell 81, 2013-2030.e9. PMID: 33773106

참고사항

For research use only

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참고 문헌

데이터시트

Anti-Human ATG4D Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-Human ATG4D Polyclonal Antibody (HC229014)

개요

이미지

참고 문헌

데이터시트

SDS