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| 카탈로그 번호 | HW696014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 설명 |
Anti-Human ATP5PO Polyclonal Antibody (HW696014) is a rabbit polyclonal antibody detecting ATP5PO in ELISA, IHC, WB. Suitable for Human.
Highlights
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| 종 반응성 | Human | ||||||||
| 응용 분야 | ELISA, IHC, WB | ||||||||
| 숙주 종 | Rabbit | ||||||||
| 클론성 | Polyclonal | ||||||||
| 이소타입 | IgG | ||||||||
| 면역원 | E. coli - derived recombinant Human ATP5PO (Gly36-Val213). | ||||||||
| 타겟 | ATP synthase peripheral stalk subunit OSCP, ATP5O, Oligomycin sensitivity conferral protein, ATP5PO, OSCP, ATPO, ATP synthase subunit O, mitochondrial | ||||||||
| 정제 | Purified by antigen affinity column. | ||||||||
| 등록번호 | P48047 | ||||||||
| 형태 | Liquid | ||||||||
| 저장 완충액 | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
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| 제품 사용 정보 |
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| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 배경 | ATP synthase peripheral stalk subunit OSCP, mitochondrial (ATP5PO) is a ~23 kDa protein. Subunit OSCP, of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. ATP synthase complex consist of a soluble F(1) head domain - the catalytic core - and a membrane F(1) domain - the membrane proton channel. These two domains are linked by a central stalk rotating inside the F(1) region and a stationary peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation (Probable). In vivo, can only synthesize ATP although its ATP hydrolase activity can be activated artificially in vitro. 1. Lai, Y. et al. (2023) Molecular cell 83, 2137-2147.e4. PMID: 37244256 | ||||||||
| 참고사항 | For research use only. |
Western blot analysis was performed using anti-ATP5PO polyclonal antibody at 1ug/mL on various samples.
Lane 1: A549 cell lysate
Lane 2: SKOV3 cell lysate
Lane 3: MCF-7 cell lysate
Lane 4: Jurkat cell lysate
Lane 5: HepG2 cell lysate
Lane 6: 293T cell lysate
Lane 7: Hela cell lysate
Lane 8: Mouse brain lysate
Lane 9: Mouse heart lysate
Lane 10: Mouse liver lysate
Lane11: Rat brain lysate
Lane12: Rat heart lysate
Lane13: Rat liver lysate
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