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| 카탈로그 번호 | HB120014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 종 반응성 | Human, Mouse, Rat | ||||||||
| 응용 분야 | ELISA, IHC, WB | ||||||||
| 숙주 종 | Rabbit | ||||||||
| 클론성 | Polyclonal | ||||||||
| 이소타입 | IgG | ||||||||
| 면역원 | E. coli - derived recombinant Human ATP6V1B2 (Ser51-His511). | ||||||||
| 타겟 | Endomembrane proton pump 58 kDa subunit, ATP6B2, V-type proton ATPase subunit B, brain isoform, HO57, VPP3, Vacuolar proton pump subunit B 2, ATP6V1B2, V-ATPase subunit B 2 | ||||||||
| 정제 | Purified by antigen affinity column. | ||||||||
| 등록번호 | P21281 | ||||||||
| 형태 | Liquid | ||||||||
| 저장 완충액 | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
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| 제품 사용 정보 |
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| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 배경 | V-type proton ATPase subunit B, brain isoform (ATP6V1B2) is a ~56 kDa protein. Non-catalytic subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment. In renal intercalated cells, can partially compensate the lack of ATP6V1B1 and mediate secretion of protons (H+) into the urine under base-line conditions but not in conditions of acid load. 1. Wang, L. et al. (2020) Molecular cell 80, 501-511.e3. PMID: 33065002 2. Vasanthakumar, T. et al. (2020) Trends in biochemical sciences 45, 295-307. PMID: 32001091 | ||||||||
| 참고사항 | For research use only. |
Western blot analysis was performed using anti-ATP6V1B2 polyclonal antibody at 1μg/ml on various samples.
Lane 1: 293T cell lysate
Lane 2: Mouse kidney lysate
Lane 3: Mouse brain lysate
Lane 4: Rat kidney lysate
Lane 5: Rat brain lysate
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