Anti-Human Axl Recombinant Antibody (SAA0128) (HB709207)

Name: Anti-Human Axl Recombinant Antibody (SAA0128)
Brand: abinScience
SKU: HB709207
Price: 191 USD
Availability: InStock

데이터시트 제품 공유 정보 복사

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개요
이미지
참고 문헌
데이터시트
SDS

개요

카탈로그 번호	HB709207
종 반응성	Human
응용 분야	FCM, WB
숙주 종	Mouse
클론성	Monoclonal
이소타입	IgG2a, kappa
클론 ID	SAA0128
테스트된 응용 분야	FCM
타겟	UFO, AXL oncogene, Tyrosine-protein kinase receptor UFO, AXL
내독소 수준	Please contact with the lab for this information.
순도	>95% as determined by SDS-PAGE.
정제	Protein A/G purified from cell culture supernatant.
등록번호	P30530
형태	Liquid
저장 완충액	0.01M PBS pH 7.4 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.
안정성 및 저장	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
배경	Tyrosine-protein kinase receptor UFO (AXL) is a ~98 kDa protein. Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding growth factor GAS6 and which is thus regulating many physiological processes including cell survival, cell proliferation, migration and differentiation. Ligand binding at the cell surface induces dimerization and autophosphorylation of AXL. Following activation by ligand, AXL binds and induces tyrosine phosphorylation of PI3-kinase subunits PIK3R1, PIK3R2 and PIK3R3; but also GRB2, PLCG1, LCK and PTPN11. Other downstream substrate candidates for AXL are CBL, NCK2, SOCS1 and TNS2. Recruitment of GRB2 and phosphatidylinositol 3 kinase regulatory subunits by AXL leads to the downstream activation of the AKT kinase.
참고사항	For research use only.

이미지

Flow Cytometry

Flow-cytometry using anti-human AXL antibody.HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human AXL antibody monoclonal antibody (Catalog # HB709207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human AXL antibody.U251 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human AXL antibody monoclonal antibody (Catalog # HB709207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.