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| 카탈로그 번호 | HF757023 | ||||
|---|---|---|---|---|---|
| 종 반응성 | Human | ||||
| 응용 분야 | ELISA, IP | ||||
| 숙주 종 | Mouse | ||||
| 클론성 | Monoclonal | ||||
| 이소타입 | IgG2a | ||||
| 클론 ID | SAA2122 | ||||
| 타겟 | BRD4, HUNK1, Bromodomain-containing protein 4, Protein HUNK1 | ||||
| 내독소 수준 | Please contact with the lab for this information. | ||||
| 순도 | >95% as determined by SDS-PAGE. | ||||
| 정제 | Protein A/G purified from cell culture supernatant. | ||||
| 등록번호 | O60885 | ||||
| RRID | Anti-Human BRD4 Antibody (SAA2122) (abinScience Cat# HF757023, RRID:AB_3729474) |
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| 형태 | Liquid | ||||
| 저장 완충액 | 0.01M PBS pH 7.4 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
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| 제품 사용 정보 |
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| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. | ||||
| 배경 | Bromodomain-containing protein 4 (BRD4) is a ~152 kDa protein. Chromatin reader protein that recognizes and binds acetylated histones and plays a key role in transmission of epigenetic memory across cell divisions and transcription regulation. Remains associated with acetylated chromatin throughout the entire cell cycle and provides epigenetic memory for postmitotic G1 gene transcription by preserving acetylated chromatin status and maintaining high-order chromatin structure. During interphase, plays a key role in regulating the transcription of signal-inducible genes by associating with the P-TEFb complex and recruiting it to promoters. Also recruits P-TEFb complex to distal enhancers, so called anti-pause enhancers in collaboration with JMJD6. BRD4 and JMJD6 are required to form the transcriptionally active P-TEFb complex by displacing negative regulators such as HEXIM1 and 7SKsnRNA complex from P-TEFb, thereby transforming it into an active form that can then phosphorylate the C-terminal domain (CTD) of RNA polymerase II. 1. Filippakopoulos, P. et al. (2010) Nature 468, 1067-73. PMID: 20871596 2. Zhang, W. et al. (2012) The Journal of biological chemistry 287, 43137-55. PMID: 23086925 3. Wu, SY. et al. (2013) Molecular cell 49, 843-57. PMID: 23317504 4. Konuma, T. et al. (2017) Scientific reports 7, 16272. PMID: 29176719 5. Olley, G. et al. (2018) Nature genetics 50, 329-332. PMID: 29379197 6. Wang, R. et al. (2012) The Journal of biological chemistry 287, 10738-52. PMID: 22334664 7. Patel, MC. et al. (2013) Molecular and cellular biology 33, 2497-507. PMID: 23589332 8. Jang, MK. et al. (2005) Molecular cell 19, 523-34. PMID: 16109376 9. Yang, Z. et al. (2005) Molecular cell 19, 535-45. PMID: 16109377 | ||||
| 참고사항 | For research use only. |
SDS-PAGE for Anti-Human BRD4 Antibody (SAA2122)
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