Anti-Human CASP3/Caspase-3 Polyclonal Antibody (HW555014)

Name: Anti-Human CASP3/Caspase-3 Polyclonal Antibody
Brand: abinScience
SKU: HW555014
Price: 96 USD
Availability: InStock

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개요
이미지
참고 문헌
데이터시트
SDS

개요

카탈로그 번호

HW555014

설명

Anti-Human CASP3/Caspase-3 Polyclonal Antibody (HW555014) is a rabbit polyclonal antibody detecting Caspase-3 in ELISA, IHC, WB. Suitable for Human, Rat, Pig, Dog, and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

종 반응성

Human, Rat

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human CASP3/Caspase-3 (Ser29-His277).

타겟

Caspase-3, CPP-32, CPP32, CASP-3, SREBP cleavage activity 1, CASP3, Apopain, SCA-1, Cysteine protease CPP32, Protein Yama

내독소 수준

Please contact with the lab for this information.

정제

Purified by antigen affinity column.

등록번호

P42574, P55213, Q95ND5, Q8MKI5, P70677, Q8MJU1, Q8MJC3

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Caspase-3 (CASP3) is a ~31 kDa protein. Thiol protease that acts as a major effector caspase involved in the execution phase of apoptosis. Following cleavage and activation by initiator caspases (CASP8, CASP9 and/or CASP10), mediates execution of apoptosis by catalyzing cleavage of many proteins. At the onset of apoptosis, it proteolytically cleaves poly(ADP-ribose) polymerase PARP1 at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9 (CASP6, CASP7 and CASP9, respectively).

1. Walsh, JG. et al. (2008) Proceedings of the National Academy of Sciences of the United States of America 105, 12815-9. PMID: 18723680

2. Nakatsumi, H. et al. (2010) The Journal of biological chemistry 285, 25418-25. PMID: 20566630

3. Thomsen, ND. et al. (2013) Proceedings of the National Academy of Sciences of the United States of America 110, 8477-82. PMID: 23650375

4. Peng, T. et al. (2022) Molecular cell 82, 1806-1820.e8. PMID: 35338844

5. Liu, Y. et al. (2022) mBio 13, e0069022. PMID: 35446120

6. Nicholson, DW. et al. (1995) Nature 376, 37-43. PMID: 7596430

10. Shi, X. et al. (2023) Nature 624, 442-450. PMID: 37993714

참고사항

For research use only.

이미지

Western blot

Western blot analysis was performed using anti-CASP3 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Jurkat cell lysate
Lane 2: Hela cell lysate

참고 문헌

데이터시트

Anti-Human CASP3/Caspase-3 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-Human CASP3/Caspase-3 Polyclonal Antibody (HW555014)

개요

이미지

참고 문헌

데이터시트

SDS

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