Anti-Human CCL20/MIP-3-alpha Recombinant Antibody (SAA0277) (HX131013)

Name: Anti-Human CCL20/MIP-3-alpha Recombinant Antibody (SAA0277)
Brand: abinScience
SKU: HX131013
Price: 110 USD
Availability: InStock

데이터시트 제품 공유 정보 복사

가격(USD):

사양:

20ug
50ug
100ug
1mg

수량:

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개요
이미지
참고 문헌
데이터시트
SDS

개요

카탈로그 번호

HX131013

종 반응성

Human

응용 분야

Blocking, ELISA, FCM

숙주 종

Mouse

클론성

Monoclonal

이소타입

IgG1, kappa

클론 ID

SAA0277

타겟

Beta-chemokine exodus-1, SCYA20, LARC, C-C motif chemokine 20, Liver and activation-regulated chemokine, Small-inducible cytokine A20, MIP-3-alpha, CCL20, MIP3A, Macrophage inflammatory protein 3 alpha, CC chemokine LARC

내독소 수준

Please contact with the lab for this information.

순도

>95% as determined by SDS-PAGE.

정제

Protein A/G purified from cell culture supernatant.

등록번호

P78556

형태

Liquid

저장 완충액

0.01M PBS pH 7.4

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:2000-1:20000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

배경

C-C motif chemokine 20 (CCL20/MIP-3-alpha) is a ~10 kDa protein. Acts as a ligand for C-C chemokine receptor CCR6. Signals through binding and activation of CCR6 and induces a strong chemotactic response and mobilization of intracellular calcium ions. The ligand-receptor pair CCL20-CCR6 is responsible for the chemotaxis of dendritic cells (DC), effector/memory T-cells and B-cells and plays an important role at skin and mucosal surfaces under homeostatic and inflammatory conditions, as well as in pathology, including cancer and various autoimmune diseases. CCL20 acts as a chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. Involved in the recruitment of both the pro-inflammatory IL17 producing helper T-cells (Th17) and the regulatory T-cells (Treg) to sites of inflammation.

1. Schutyser, E. et al. (2000) Journal of immunology (Baltimore, Md. : 1950) 165, 4470-7. PMID: 11035086

2. Nelson, RT. et al. (2001) Genomics 73, 28-37. PMID: 11352563

3. Röhrl, J. et al. (2010) The Journal of biological chemistry 285, 7028-34. PMID: 20068036

4. Ito, T. et al. (2011) Experimental cell research 317, 613-9. PMID: 21376174

5. Hieshima, K. et al. (1997) The Journal of biological chemistry 272, 5846-53. PMID: 9038201

6. Caballero-Campo, P. et al. (2014) Journal of cellular physiology 229, 68-78. PMID: 23765988

7. Diao, R. et al. (2014) Science translational medicine 6, 249ra108. PMID: 25122636

8. Hromas, R. et al. (1997) Blood 89, 3315-22. PMID: 9129037

참고사항

For research use only.

Isotype Control

Mouse IgG1, kappa Recombinant Isotype Control Antibody (MOPC-21) (MV080207)

이미지

Flow Cytometry

Flow-cytometry using anti-human CCL20 antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human CCL20 antibody monoclonal antibody (Catalog # HX131013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE

SDS-PAGE for Anti-Human CCL20/MIP-3-alpha Antibody (SAA0277).