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Anti-Human CD276/B7-H3 Polyclonal Antibody (HV612014)

Anti-Human CD276/B7-H3 Polyclonal Antibody
Anti-Human CD276/B7-H3 Polyclonal Antibody
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사양:
  • 20ug
  • 50ug
  • 100ug
수량:
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  • 개요

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  • 데이터시트

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개요
카탈로그 번호HV612014
설명
Anti-Human CD276 Polyclonal Antibody (HV612014) is a rabbit polyclonal antibody detecting CD276 in ELISA, IHC, WB. Suitable for Rattus norvegicus, Mus musculus, and Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
종 반응성Human, Rat
응용 분야ELISA, IHC, WB
숙주 종Rabbit
클론성Polyclonal
이소타입IgG
면역원 E. coli - derived recombinant Human CD276 (Thr47-Ser219).
테스트된 응용 분야 WB
타겟 4Ig-B7-H3, B7 homolog 3, B7-H3, B7H3, CD276, CD276 antigen, Costimulatory molecule
내독소 수준 Please contact with the lab for this information.
순도 >90% as determined by SDS-PAGE.
정제 Purified by antigen affinity column.
등록번호 Q5ZPR3
형태 Liquid
저장 완충액 0.01M PBS, pH 7.4, 50% glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보
응용 분야 희석
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
안정성 및 저장 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
대체 이름CD276 antigen, 4Ig-B7-H3, B7 homolog 3, B7-H3, Costimulatory molecule, CD276, CD276, B7H3, PSEC0249, UNQ309/PRO352
배경

CD276 antigen is a ~57 kDa protein. May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses.

참고사항 For research use only.
이미지
  • Anti-Human CD276/B7-H3 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-CD276 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Hela cell lysate
    Lane 2: Lncap cell lysate
    Lane 3: U2OS cell lysate
    Lane 4: A431 cell lysate
    Lane 5: HepG2 cell lysate
    Lane 6: 293T cell lysate
    Lane 7: MCF-7 cell lysate

참고 문헌
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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