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Anti-Human Fc gamma RIIA/CD32a Recombinant Antibody (IV.3) (HY139107)

Anti-Human Fc gamma RIIA/CD32a Recombinant Antibody (IV.3)
Anti-Human Fc gamma RIIA/CD32a Recombinant Antibody (IV.3)
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  • 개요

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개요
카탈로그 번호HY139107
종 반응성Human
응용 분야ELISA, FCM, IF, IP
숙주 종Human
클론성Monoclonal
이소타입IgG1, kappa
클론 IDIV.3
테스트된 응용 분야 FCM
타겟 Fc-gamma RII-a, IgG Fc receptor II-a, IGFR2, FCG2, CDw32, FCGR2A1, FCGR2A, FcRII-a, CD32, Low affinity immunoglobulin gamma Fc region receptor II-a, Fc-gamma-RIIa
내독소 수준 Please contact with the lab for this information.
순도 >95% as determined by SDS-PAGE.
정제 Protein A/G purified from cell culture supernatant.
등록번호 P12318
형태 Liquid
저장 완충액 0.01M PBS pH 7.4

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

안정성 및 저장 Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
배경

Low affinity immunoglobulin gamma Fc region receptor II-a (CD32/FCGR2A) is a ~35 kDa protein. Binds to the Fc region of immunoglobulins gamma. Low affinity receptor. By binding to IgG it initiates cellular responses against pathogens and soluble antigens. Promotes phagocytosis of opsonized antigens.

참고사항 For research use only.
이미지
  • Anti-Human Fc gamma RIIA/CD32a Recombinant Antibody (IV.3)

    Flow Cytometry

    Flow-cytometry using anti-human CD32 antibody.CD32 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD32 antibody monoclonal antibody (Catalog # HY139107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.

참고 문헌
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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