Anti-Human E-Cadherin Recombinant Antibody (67A4) (HY460107)

Name: Anti-Human E-Cadherin Recombinant Antibody (67A4)
Brand: abinScience
SKU: HY460107
Price: 191 USD
Availability: InStock

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개요
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참고 문헌
데이터시트
SDS

개요

카탈로그 번호	HY460107
종 반응성	Human
응용 분야	FCM, IF, IP
숙주 종	Mouse
클론성	Monoclonal
이소타입	IgG1, kappa
클론 ID	67A4
테스트된 응용 분야	FCM
타겟	CD324, Uvomorulin, Epithelial cadherin, E-cadherin, CDHE, CAM 120/80, UVO, CDH1, Cadherin-1
내독소 수준	Please contact with the lab for this information.
순도	>95% as determined by SDS-PAGE.
정제	Protein A/G purified from cell culture supernatant.
등록번호	P12830
형태	Liquid
저장 완충액	0.01M PBS pH 7.4 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.
안정성 및 저장	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
배경	Cadherin-1 (CD324/CDH1) is a ~97 kDa protein. Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Promotes organization of radial actin fiber structure and cellular response to contractile forces, via its interaction with AMOTL2 which facilitates anchoring of radial actin fibers to CDH1 junction complexes at the cell membrane. Plays a role in the early stages of desmosome cell-cell junction formation via facilitating the recruitment of DSG2 and DSP to desmosome plaques. 1. Meigs, TE. et al. (2002) The Journal of biological chemistry 277, 24594-600. PMID: 11976333 2. Shafraz, O. et al. (2018) eLife 7. PMID: 29999492
참고사항	For research use only.

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Bioactivity

Detects CD324/CDH1 in indirect ELISAs.
Flow Cytometry

Flow-cytometry using anti-human CD324 antibody.HT-29 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD324 antibody monoclonal antibody (Catalog # HY460107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human CD324 antibody.MCF7 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD324 antibody monoclonal antibody (Catalog # HY460107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using anti-human CD324 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD324 monoclonal antibody (Catalog: HY460107) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.