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| 카탈로그 번호 | HB185013 | ||||||
|---|---|---|---|---|---|---|---|
| 브랜드 | abinScience | ||||||
| 설명 |
Anti-Human CD33 Nanobody (SAA2052) [SAA2052] (HB185013) is a alpaca monoclonal antibody detecting CD33 in ELISA, FCM. Suitable for Human and Cynomolgus.
Highlights
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| 종 반응성 | Human, Monkey | ||||||
| 응용 분야 | ELISA, FCM, WB | ||||||
| 숙주 종 | Alpaca | ||||||
| 클론성 | Monoclonal | ||||||
| 이소타입 | VHH-8His-Cys-tag | ||||||
| 클론 ID | SAA2052 | ||||||
| 테스트된 응용 분야 | WB | ||||||
| 타겟 | Myeloid cell surface antigen CD33, Sialic acid-binding Ig-like lectin 3, Siglec-3, CD33, gp67, SIGLEC3 | ||||||
| 내독소 수준 | Please contact with the lab for this information. | ||||||
| 순도 | >95% as determined by SDS-PAGE. | ||||||
| 정제 | Purified by Nickel column. | ||||||
| 등록번호 | P20138 | ||||||
| 형태 | Liquid | ||||||
| 저장 완충액 | 0.01M PBS pH 7.4 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
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| 제품 사용 정보 |
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| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||
| 배경 | Myeloid cell surface antigen CD33 is a ~39 kDa protein. Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state. Preferentially recognizes and binds alpha-2,3- and more avidly alpha-2,6-linked sialic acid-bearing glycans. Upon engagement of ligands such as C1q or syalylated glycoproteins, two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) located in CD33 cytoplasmic tail are phosphorylated by Src-like kinases such as LCK. These phosphorylations provide docking sites for the recruitment and activation of protein-tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2. In turn, these phosphatases regulate downstream pathways through dephosphorylation of signaling molecules. CD33 is the therapeutic target of gemtuzumab ozogamicin (Mylotarg). 1. Vitale, C. et al. (1999) Proceedings of the National Academy of Sciences of the United States of America 96, 15091-6. PMID: 10611343 2. Vitale, C. et al. (2001) Proceedings of the National Academy of Sciences of the United States of America 98, 5764-9. PMID: 11320212 3. Lajaunias, F. et al. (2005) European journal of immunology 35, 243-51. PMID: 15597323 4. Freeman, SD. et al. (1995) Blood 85, 2005-12. PMID: 7718872 5. Paul, SP. et al. (2000) Blood 96, 483-90. PMID: 10887109 6. Son, M. et al. (2017) Scientific reports 7, 270. PMID: 28325905 7. Taylor, VC. et al. (1999) The Journal of biological chemistry 274, 11505-12. PMID: 10206955 8. Ulyanova, T. et al. (1999) European journal of immunology 29, 3440-9. PMID: 10556798 | ||||||
| 참고사항 | For research use only. |
SDS-PAGE for Anti-Human CD33 Recombinant Nanobody (SAA2052)
Detects CD33 (Catalog No: HB185011) in indirect ELISA.
Western blot analysis was performed using anti-CD33 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human CD33 (Catalog No: HB185011)
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