Anti-Human CD3 epsilon Recombinant Nanobody (SAA2242) (HY057043)

Name: Anti-Human CD3 epsilon Recombinant Nanobody (SAA2242)
Brand: abinScience
SKU: HY057043
Price: 126 USD
Availability: InStock

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20ug
50ug
100ug
1mg

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개요
이미지
참고 문헌
데이터시트
SDS

개요

카탈로그 번호

HY057043

설명

Anti-Human CD3E Nanobody (SAA2242) [SAA2242] (HY057043) is a alpaca monoclonal antibody detecting CD3e in ELISA, FCM. Suitable for Human.

Highlights

●Flow Cytometry — Validated for flow cytometry applications.

종 반응성

Human

응용 분야

ELISA, FCM

숙주 종

Alpaca

클론성

Monoclonal

이소타입

VHH-His

클론 ID

SAA2242

테스트된 응용 분야

FCM

타겟

CD3E, CD3e, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain, T3E

내독소 수준

< 10 EU/mg

순도

>95% as determined by SDS-PAGE.

정제

Purified by Nickel column.

등록번호

P07766

형태

Liquid

저장 완충액

0.01M PBS pH 7.4.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:2000-1:20000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

T-cell surface glycoprotein CD3 epsilon chain (CD3E) is a ~23 kDa protein. Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD247/CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. CD3E ITAM phosphorylation creates docking sites for the protein kinase ZAP70 leading to ZAP70 phosphorylation and its conversion into a catalytically active enzyme. CD3E is the therapeutic target of blinatumomab (Blincyto).

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1. Gimferrer, I. et al. (2004) Journal of immunology (Baltimore, Md. : 1950) 173, 2262-70. PMID: 15294938

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2. de Saint Basile, G. et al. (2004) The Journal of clinical investigation 114, 1512-7. PMID: 15546002

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3. Barber, EK. et al. (1989) Proceedings of the National Academy of Sciences of the United States of America 86, 3277-81. PMID: 2470098

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4. Du, J. et al. (2025) Cell 188, 4025-4042.e20. PMID: 40592325

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5. Soudais, C. et al. (1993) Nature genetics 3, 77-81. PMID: 8490660

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참고사항

For research use only.

이미지

SDS-PAGE

SDS-PAGE for Anti-Human CD3E Nanobody (SAA2242)
Bioactivity

Detects recombinant human CD3E (Catalog No: HY057031) in indirect ELISA.
Flow cytometry

Flow cytometry analysis was performed using anti-human CD3 monoclonal antibody. Jurkat cells were incubated with an irrelevant antibody (blue histogram) or FITC-conjugated anti-human CD3 monoclonal antibody (Catalog # HY057043, green histogram) at a concentration of 5 µg/mL for 30 min at room temperature. After washing, cells were directly analyzed on a NovoCyte Flow Cytometer.