Anti-Human CD45 Recombinant Nanobody (2H5) (HY195013)

Name: Anti-Human CD45 Recombinant Nanobody (2H5)
Brand: abinScience
SKU: HY195013
Price: 126 USD
Availability: InStock

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사양:

20ug
50ug
100ug
1mg

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개요
이미지
참고 문헌
데이터시트
SDS

개요

카탈로그 번호

HY195013

설명

Anti-Human CD45/PTPRC Nanobody (2H5) [2H5] (HY195013) is a alpaca monoclonal antibody detecting Ptprc in ELISA, SPR. Suitable for Human.

종 반응성

Human

응용 분야

ELISA, SPR

숙주 종

Alpaca

클론성

Monoclonal

이소타입

VHH-8His-Cys-tag

클론 ID

2H5

타겟

Receptor-type tyrosine-protein phosphatase C, 3.1.3.48, Leukocyte common antigen, L-CA, Lymphocyte antigen 5, Ly-5, T200, CD45, Ptprc, Ly-5

내독소 수준

Please contact with the lab for this information.

순도

>95% as determined by SDS-PAGE.

정제

Protein A/G purified from cell culture supernatant.

등록번호

P06800

형태

Liquid

저장 완충액

0.01M PBS pH 7.4

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:2000-1:20000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Receptor-type tyrosine-protein phosphatase C (CD45/PTPRC) is a ~144 kDa protein. Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity.

참고사항

For research use only.

이미지

SDS-PAGE

SDS-PAGE for Anti-Human CD45/PTPRC Nanobody (2H5)
Bioactivity

Detects recombinant CD45 (Catalog No: HY195011) in indirect ELISA.
Immunofluorescence

Immunofluorescence staining of Jurkat with Alpaca monoclonal antibody (20ug/ml, overnight at 4°C), followed by Alexa Fluor-488-conjugated secondary antibody (6.5ug/ml, 50minutes at room temperature). The target protein was detected in the Cell membrane.

참고 문헌

데이터시트

Anti-Human CD45 Recombinant Nanobody (2H5).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-Human CD45 Recombinant Nanobody (2H5) (HY195013)

개요

이미지

참고 문헌

데이터시트

SDS

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