Anti-Human B7-2/CD86 Recombinant Antibody (SAA1397) (HW776207)

Name: Anti-Human B7-2/CD86 Recombinant Antibody (SAA1397)
Brand: abinScience
SKU: HW776207
Price: 191 USD
Availability: InStock

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개요
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참고 문헌
데이터시트
SDS

개요

카탈로그 번호	HW776207
종 반응성	Human
응용 분야	ELISA, FCM
숙주 종	Mouse
클론성	Monoclonal
이소타입	IgG2a, kappa
클론 ID	SAA1397
테스트된 응용 분야	FCM
타겟	BU63, B70, CD28LG2, CTLA-4 counter-receptor B7.2, Activation B7-2 antigen, T-lymphocyte activation antigen CD86, CD86, FUN-1
내독소 수준	Please contact with the lab for this information.
순도	>95% as determined by SDS-PAGE.
정제	Protein A/G purified from cell culture supernatant.
등록번호	P42081
형태	Liquid
저장 완충액	0.01M PBS, pH 7.4, 0.09% Sodium azide. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.
안정성 및 저장	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
배경	T-lymphocyte activation antigen CD86 (B7-2) is a ~37 kDa protein. Costimulatory molecule that belongs to the immunoglobulin superfamily that plays an important role in T-lymphocyte activation. Acts as the primary auxiliary signal augmenting the MHC/TCR signal in naive T-cells by acting as a ligand for the CD28 receptor which is constitutively expressed on the cell surface of T-cells. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Also involved in the regulation of B cells function, plays a role in regulating the level of IgG(1) produced. Upon CD40 engagement, activates NF-kappa-B signaling pathway via phospholipase C and protein kinase C activation. 1. Collins, AV. et al. (2002) Immunity 17, 201-10. PMID: 12196291 2. Freeman, GJ. et al. (1993) Science (New York, N.Y.) 262, 909-11. PMID: 7694363 3. Lanier, LL. et al. (1995) Journal of immunology (Baltimore, Md. : 1950) 154, 97-105. PMID: 7527824 4. Schwartz, JC. et al. (2001) Nature 410, 604-8. PMID: 11279501
참고사항	For research use only.

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Bioactivity

Detects Human CD86/B7-2 in indirect ELISAs.
Flow Cytometry

Flow-cytometry using anti-human CD86 antibody.Daudi cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD86 antibody monoclonal antibody (Catalog # HW776207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human CD86 antibody.K562 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD86 antibody monoclonal antibody (Catalog # HW776207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.