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| 카탈로그 번호 | HB064014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 종 반응성 | Human | ||||||||
| 응용 분야 | ELISA, IHC, WB | ||||||||
| 숙주 종 | Rabbit | ||||||||
| 클론성 | Polyclonal | ||||||||
| 이소타입 | IgG | ||||||||
| 면역원 | E. coli - derived recombinant Human CD88/C5AR1 (Arg175-Arg206). | ||||||||
| 타겟 | CD88, C5AR1, C5R1, C5AR, C5a-R, C5aR, C5a anaphylatoxin chemotactic receptor 1, C5a anaphylatoxin chemotactic receptor | ||||||||
| 정제 | Purified by antigen affinity column. | ||||||||
| 등록번호 | P21730 | ||||||||
| 형태 | Liquid | ||||||||
| 저장 완충액 | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
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| 제품 사용 정보 |
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| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 배경 | C5a anaphylatoxin chemotactic receptor 1 (CD88/C5AR1) is a ~39 kDa protein. Receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a, stimulating chemotaxis, granule enzyme release, intracellular calcium release and superoxide anion production. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as adenylate cyclase. C5AR1 is coupled to G(i)/G(o) (GNAI1 or GNAO1) G alpha proteins and mediates inhibition of adenylate cyclase. The ligand interacts with at least two sites on the receptor: a high-affinity site on the extracellular N-terminus, and a second site in the transmembrane region which activates downstream signaling events. 1. Christophe, T. et al. (2000) The Journal of biological chemistry 275, 1656-64. PMID: 10636859 2. Postma, B. et al. (2004) Journal of immunology (Baltimore, Md. : 1950) 172, 6994-7001. PMID: 15153520 3. Gerard, NP. et al. (1991) Nature 349, 614-7. PMID: 1847994 4. Robertson, N. et al. (2018) Nature 553, 111-114. PMID: 29300009 5. Feng, Y. et al. (2023) Cell research 33, 312-324. PMID: 36806352 6. Monk, PN. et al. (1995) The Journal of biological chemistry 270, 16625-9. PMID: 7622471 7. DeMartino, JA. et al. (1994) The Journal of biological chemistry 269, 14446-50. PMID: 8182049 8. Chen, Z. et al. (1998) The Journal of biological chemistry 273, 10411-9. PMID: 9553099 9. Yadav, MK. et al. (2023) Cell 186, 4956-4973.e21. PMID: 37852260 | ||||||||
| 참고사항 | For research use only. |
Western blot analysis was performed using anti-C5AR1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: U937 cell lysate
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