Anti-Human CENPE Polyclonal Antibody (HX024014)

Name: Anti-Human CENPE Polyclonal Antibody
Brand: abinScience
SKU: HX024014
Price: 168 USD
Availability: InStock

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개요
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참고 문헌
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개요

카탈로그 번호

HX024014

설명

Anti-Human CENPE Polyclonal Antibody (HX024014) is a rabbit polyclonal antibody detecting CENPE in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

종 반응성

Human

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human CENPE (Ala2450-Arg2590).

타겟

CENP-E, CENPE, Kinesin-related protein CENPE, Centromere-associated protein E, Centromere protein E, Kinesin-7

정제

Purified by antigen affinity column.

등록번호

Q02224

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Centromere-associated protein E (CENPE) is a ~316 kDa protein. Microtubule plus-end-directed kinetochore motor which plays an important role in chromosome congression, microtubule-kinetochore conjugation and spindle assembly checkpoint activation. Drives chromosome congression (alignment of chromosomes at the spindle equator resulting in the formation of the metaphase plate) by mediating the lateral sliding of polar chromosomes along spindle microtubules towards the spindle equator and by aiding the establishment and maintenance of connections between kinetochores and spindle microtubules. The transport of pole-proximal chromosomes towards the spindle equator is favored by microtubule tracks that are detyrosinated. Acts as a processive bi-directional tracker of dynamic microtubule tips; after chromosomes have congressed, continues to play an active role at kinetochores, enhancing their links with dynamic microtubule ends. Suppresses chromosome congression in NDC80-depleted cells and contributes positively to congression only when microtubules are stabilized.

1. Shrestha, RL. et al. (2013) Current biology : CB 23, 1514-26. PMID: 23891108

2. Bancroft, J. et al. (2015) Journal of cell science 128, 171-84. PMID: 25395579

3. Thrower, DA. et al. (1995) The EMBO journal 14, 918-26. PMID: 7889940

4. Barisic, M. et al. (2015) Science (New York, N.Y.) 348, 799-803. PMID: 25908662

5. Gudimchuk, N. et al. (2013) Nature cell biology 15, 1079-1088. PMID: 23955301

6. Iemura, K. et al. (2015) Nature communications 6, 6447. PMID: 25743205

7. Liu, D. et al. (2007) The Journal of biological chemistry 282, 21415-24. PMID: 17535814

참고사항

For research use only.

이미지

참고 문헌

데이터시트

Anti-Human CENPE Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-Human CENPE Polyclonal Antibody (HX024014)

개요

이미지

참고 문헌

데이터시트

SDS