Anti-Human CFH Recombinant Nanobody (SAA2038) (HY505023)

Name: Anti-Human CFH Recombinant Nanobody (SAA2038)
Brand: abinScience
SKU: HY505023
Price: 126 USD
Availability: InStock

데이터시트 제품 공유 정보 복사

가격(USD):

사양:

20ug
50ug
100ug
1mg

수량:

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개요
이미지
참고 문헌
데이터시트
SDS

개요

카탈로그 번호

HY505023

설명

Anti-Human CFH Nanobody (SAA2038) [SAA2038] (HY505023) is a alpaca monoclonal antibody detecting CFH in ELISA. Suitable for Human.

종 반응성

Human

응용 분야

ELISA

숙주 종

Alpaca

클론성

Monoclonal

이소타입

VHH-8His-Cys-tag

클론 ID

SAA2038

타겟

Complement factor H, CFH, H factor 1, HF1, HF, HF2

내독소 수준

Please contact with the lab for this information.

순도

>95% as determined by SDS-PAGE.

정제

Purified by Nickel column.

등록번호

P08603

형태

Liquid

저장 완충액

0.01M PBS pH 7.4

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:2000-1:20000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Complement factor H (CFH) is a ~139 kDa protein. Glycoprotein that plays an essential role in maintaining a well-balanced immune response by modulating complement activation. Acts as a soluble inhibitor of complement, where its binding to self markers such as glycan structures prevents complement activation and amplification on cell surfaces. Accelerates the decay of the complement alternative pathway (AP) C3 convertase C3bBb, thus preventing local formation of more C3b, the central player of the complement amplification loop. As a cofactor of the serine protease factor I, CFH also regulates proteolytic degradation of already-deposited C3b. In addition, mediates several cellular responses through interaction with specific receptors.

1. Kajander, T. et al. (2011) Proceedings of the National Academy of Sciences of the United States of America 108, 2897-902. PMID: 21285368

2. Morgan, HP. et al. (2011) Nature structural & molecular biology 18, 463-70. PMID: 21317894

3. Blaum, BS. et al. (2015) Nature chemical biology 11, 77-82. PMID: 25402769

4. Wu, J. et al. (2009) Nature immunology 10, 728-33. PMID: 19503104

5. Kennedy, AT. et al. (2016) Journal of immunology (Baltimore, Md. : 1950) 196, 1239-48. PMID: 26700768

6. Hocking, HG. et al. (2008) The Journal of biological chemistry 283, 9475-87. PMID: 18252712

7. Simon, N. et al. (2013) Cell host & microbe 13, 29-41. PMID: 23332154

8. Xue, X. et al. (2017) Nature structural & molecular biology 24, 643-651. PMID: 28671664

9. Losse, J. et al. (2010) Journal of immunology (Baltimore, Md. : 1950) 184, 912-21. PMID: 20008295

10. DiScipio, RG. et al. (1998) Journal of immunology (Baltimore, Md. : 1950) 160, 4057-66. PMID: 9558116

참고사항

For research use only.

이미지

SDS-PAGE

SDS-PAGE for Anti-Human CFH Recombinant Nanobody (SAA2038)
Bioactivity

Detects recombinant CFH (Catalog No: HY505012) in indirect ELISA.
Immunofluorescence

Immunofluorescence staining of Hepg2 with Alpaca monoclonal antibody (20ug/ml, overnight at 4°C), followed by Alexa Fluor-488-conjugated secondary antibody (6.5ug/ml, 50minutes at room temperature). The target protein was detected in the Secreted.