Anti-Human CHEK1 Polyclonal Antibody (HA503014)

Name: Anti-Human CHEK1 Polyclonal Antibody
Brand: abinScience
SKU: HA503014
Price: 96 USD
Availability: InStock

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개요
이미지
참고 문헌
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SDS

개요

카탈로그 번호

HA503014

설명

Anti-Human CHEK1 Polyclonal Antibody (HA503014) is a rabbit polyclonal antibody detecting CHEK1 in ELISA, IHC, WB. Suitable for Rattus norvegicus, Gallus gallus, Mus musculus, and Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

종 반응성

Human, Mouse, Rat

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human CHEK1 (Met1-Asn266).

타겟

CHEK1

정제

Purified by antigen affinity column.

등록번호

O14757

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

대체 이름

Serine/threonine-protein kinase Chk1, 2.7.11.1, CHK1 checkpoint homolog, Cell cycle checkpoint kinase, Checkpoint kinase-1, CHEK1, CHK1

배경

Serine/threonine-protein kinase Chk1 (CHEK1) is a ~54 kDa protein. Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest and activation of DNA repair in response to the presence of DNA damage or unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. This regulation is achieved by a number of mechanisms that together help to preserve the integrity of the genome. Recognizes the substrate consensus sequence [R-X-X-S/T]. Binds to and phosphorylates CDC25A, CDC25B and CDC25C.

1. Feijoo, C. et al. (2001) The Journal of cell biology 154, 913-23. PMID: 11535615

2. Zhao, H. et al. (2002) Proceedings of the National Academy of Sciences of the United States of America 99, 14795-800. PMID: 12399544

3. Heffernan, TP. et al. (2002) Molecular and cellular biology 22, 8552-61. PMID: 12446774

4. Chen, MS. et al. (2003) Molecular and cellular biology 23, 7488-97. PMID: 14559997

5. Pichierri, P. et al. (2004) The EMBO journal 23, 1178-87. PMID: 14988723

6. Krämer, A. et al. (2004) Nature cell biology 6, 884-91. PMID: 15311285

7. Huang, X. et al. (2005) Proceedings of the National Academy of Sciences of the United States of America 102, 1065-70. PMID: 15650047

8. Sørensen, CS. et al. (2005) Nature cell biology 7, 195-201. PMID: 15665856

9. Yu, X. et al. (2020) Journal of hematology & oncology 13, 40. PMID: 32357935

참고사항

For research use only.

이미지

Western blot

Western blot analysis was performed using anti-CHEK1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: NIH3T3 cell lysate
Lane 2: PC-12 cell lysate

참고 문헌

데이터시트

Anti-Human CHEK1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-Human CHEK1 Polyclonal Antibody (HA503014)

개요

이미지

참고 문헌

데이터시트

SDS