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| 카탈로그 번호 | HC663014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 설명 |
Anti-Human CUL4A Polyclonal Antibody (HC663014) is a rabbit polyclonal antibody detecting CUL4A in ELISA, IHC, WB. Suitable for Human and Mouse.
Highlights
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| 종 반응성 | Human, Mouse | ||||||||
| 응용 분야 | ELISA, IHC, WB | ||||||||
| 숙주 종 | Rabbit | ||||||||
| 클론성 | Polyclonal | ||||||||
| 이소타입 | IgG | ||||||||
| 면역원 | E. coli - derived recombinant Human CUL4A (Arg666-Ala759). | ||||||||
| 타겟 | CUL-4A, CUL4A, Cullin-4A | ||||||||
| 정제 | Purified by antigen affinity column. | ||||||||
| 등록번호 | Q13619 | ||||||||
| 형태 | Liquid | ||||||||
| 저장 완충액 | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
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| 제품 사용 정보 |
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| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 배경 | Cullin-4A (CUL4A) is a ~87 kDa protein. Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. DCX(DET1-COP1) directs ubiquitination of JUN. 1. Higa, LA. et al. (2003) Nature cell biology 5, 1008-15. PMID: 14578910 2. Wertz, IE. et al. (2004) Science (New York, N.Y.) 303, 1371-4. PMID: 14739464 3. Hu, J. et al. (2004) Nature cell biology 6, 1003-9. PMID: 15448697 4. Nag, A. et al. (2004) Cancer research 64, 8152-5. PMID: 15548678 5. Matsuda, N. et al. (2005) DNA repair 4, 537-45. PMID: 15811626 6. Wang, H. et al. (2006) Molecular cell 22, 383-94. PMID: 16678110 7. Higa, LA. et al. (2006) Nature cell biology 8, 1277-83. PMID: 17041588 8. Han, J. et al. (2013) Cell 155, 817-29. PMID: 24209620 9. Chen, SH. et al. (2018) The EMBO journal 37. PMID: 30166453 10. Maiani, E. et al. (2021) Nature 592, 799-803. PMID: 33854232 | ||||||||
| 참고사항 | For research use only. |
Western blot analysis was performed using anti-CUL4A polyclonal antibody at 1ug/mL on various samples.
Lane 1: NIH3T3 cell lysate
Western blot analysis was performed using anti-CUL4A polyclonal antibody at 1μg/ml on various samples.
Lane 1: PC-12 cell lysate
Lane 2: Raji cell lysate
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