Anti-Human CYP1A2 Polyclonal Antibody (HY226014)

Name: Anti-Human CYP1A2 Polyclonal Antibody
Brand: abinScience
SKU: HY226014
Price: 168 USD
Availability: InStock

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100ug

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개요
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참고 문헌
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SDS

개요

카탈로그 번호

HY226014

설명

Anti-Human CYP1A2 Polyclonal Antibody (HY226014) is a rabbit polyclonal antibody detecting CYP1A2 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

종 반응성

Human

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human CYP1A2 (Ala2-Ser231).

타겟

Hydroperoxy icosatetraenoate dehydratase, Cytochrome P450-P3, Cytochrome P450 4, CYP1A2, Cholesterol 25-hydroxylase, CYPIA2, Cytochrome P(3)450, Cytochrome P450 1A2

정제

Purified by antigen affinity column.

등록번호

P05177

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Cytochrome P450 1A2 (CYP1A2) is a ~58 kDa protein. A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids, steroid hormones and vitamins. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds. Exhibits high catalytic activity for the formation of hydroxyestrogens from estrone (E1) and 17beta-estradiol (E2), namely 2-hydroxy E1 and E2. Metabolizes cholesterol toward 25-hydroxycholesterol, a physiological regulator of cellular cholesterol homeostasis.

1. Chen, H. et al. (2000) Drug metabolism and disposition: the biological fate of chemicals 28, 315-22. PMID: 10681376

2. Badawi, AF. et al. (2001) Metabolism: clinical and experimental 50, 1001-3. PMID: 11555828

3. Lee, AJ. et al. (2003) Endocrinology 144, 3382-98. PMID: 12865317

4. Lucas, D. et al. (2010) Journal of lipid research 51, 1125-33. PMID: 19965576

5. Bylund, J. et al. (1998) The Journal of pharmacology and experimental therapeutics 284, 51-60. PMID: 9435160

6. Honda, A. et al. (2011) Journal of lipid research 52, 1509-16. PMID: 21576599

참고사항

For research use only.

이미지

참고 문헌

데이터시트

Anti-Human CYP1A2 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-Human CYP1A2 Polyclonal Antibody (HY226014)

개요

이미지

참고 문헌

데이터시트

SDS