Anti-Human CYP46A1 Polyclonal Antibody (HV358014)

Name: Anti-Human CYP46A1 Polyclonal Antibody
Brand: abinScience
SKU: HV358014
Price: 168 USD
Availability: InStock

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개요
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참고 문헌
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개요

카탈로그 번호

HV358014

설명

Anti-Human CYP46A1 Polyclonal Antibody (HV358014) is a rabbit polyclonal antibody detecting CYP46A1 in ELISA, IHC, WB. Suitable for Human, Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

종 반응성

Human, Mouse

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human CYP46A1 (His45-Cys500).

타겟

Cholesterol 24-hydroxylase, Cholesterol 24-monooxygenase, CH24H, CYP46A1, Cytochrome P450 46A1, CYP46, Cholesterol 24S-hydroxylase

정제

Purified by antigen affinity column.

등록번호

Q9Y6A2

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Cholesterol 24-hydroxylase (CYP46A1) is a ~56 kDa protein. P450 monooxygenase that plays a major role in cholesterol homeostasis in the brain. Primarily catalyzes the hydroxylation (with S stereochemistry) at C-24 of cholesterol side chain, triggering cholesterol diffusion out of neurons and its further degradation. By promoting constant cholesterol elimination in neurons, may activate the mevalonate pathway and coordinate the synthesis of new cholesterol and nonsterol isoprenoids involved in synaptic activity and learning. Further hydroxylates cholesterol derivatives and hormone steroids on both the ring and side chain of these molecules, converting them into active oxysterols involved in lipid signaling and biosynthesis. Acts as an epoxidase converting cholesta-5,24-dien-3beta-ol/desmosterol into (24S),25-epoxycholesterol, an abundant lipid ligand of nuclear NR1H2 and NR1H3 receptors shown to promote neurogenesis in developing brain.

1. Lund, EG. et al. (1999) Proceedings of the National Academy of Sciences of the United States of America 96, 7238-43. PMID: 10377398

2. Mast, N. et al. (2003) Biochemistry 42, 14284-92. PMID: 14640697

3. Mast, N. et al. (2008) Proceedings of the National Academy of Sciences of the United States of America 105, 9546-51. PMID: 18621681

4. Goyal, S. et al. (2014) Journal of lipid research 55, 1933-43. PMID: 25017465

5. Bodin, K. et al. (2002) The Journal of biological chemistry 277, 31534-40. PMID: 12077124

6. Mast, N. et al. (2017) The Journal of biological chemistry 292, 4913-4924. PMID: 28190002

7. Mast, N. et al. (2010) The Journal of biological chemistry 285, 31783-95. PMID: 20667828

참고사항

For research use only.

이미지

참고 문헌

데이터시트

Anti-Human CYP46A1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-Human CYP46A1 Polyclonal Antibody (HV358014)

개요

이미지

참고 문헌

데이터시트

SDS