Anti-Human DDX21 Polyclonal Antibody (HP105014)

Name: Anti-Human DDX21 Polyclonal Antibody
Brand: abinScience
SKU: HP105014
Price: 96 USD
Availability: InStock

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개요
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참고 문헌
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SDS

개요

카탈로그 번호

HP105014

설명

Anti-Human DDX21 Polyclonal Antibody (HP105014) is a rabbit polyclonal antibody detecting DDX21 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

종 반응성

Human, Mouse, Rat

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human DDX21 (Ala187-Ile563).

타겟

DEAD box protein 21, DDX21, Gu-alpha, Nucleolar RNA helicase II, RH II/Gu, Nucleolar RNA helicase Gu, Nucleolar RNA helicase 2

정제

Purified by antigen affinity column.

등록번호

Q9NR30

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Nucleolar RNA helicase 2 (DDX21) is a ~87 kDa protein. RNA helicase that acts as a sensor of the transcriptional status of both RNA polymerase (Pol) I and II: promotes ribosomal RNA (rRNA) processing and transcription from polymerase II (Pol II). Binds various RNAs, such as rRNAs, snoRNAs, 7SK and, at lower extent, mRNAs. In the nucleolus, localizes to rDNA locus, where it directly binds rRNAs and snoRNAs, and promotes rRNA transcription, processing and modification. Required for rRNA 2'-O-methylation, possibly by promoting the recruitment of late-acting snoRNAs SNORD56 and SNORD58 with pre-ribosomal complexes. In the nucleoplasm, binds 7SK RNA and is recruited to the promoters of Pol II-transcribed genes: acts by facilitating the release of P-TEFb from inhibitory 7SK snRNP in a manner that is dependent on its helicase activity, thereby promoting transcription of its target genes.

1. Calo, E. et al. (2015) Nature 518, 249-53. PMID: 25470060

2. Song, C. et al. (2017) Genes & development 31, 1370-1381. PMID: 28790157

3. Sloan, KE. et al. (2015) Nucleic acids research 43, 553-64. PMID: 25477391

4. Westermarck, J. et al. (2002) The EMBO journal 21, 451-60. PMID: 11823437

5. Zhang, Y. et al. (2014) Breast cancer research : BCR 16, 449. PMID: 25260534

6. Valdez, BC. et al. (1997) European journal of biochemistry 250, 800-7. PMID: 9461305

9. Treiber, T. et al. (2017) Molecular cell 66, 270-284.e13. PMID: 28431233

참고사항

For research use only.

이미지

Western blot

Western blot analysis was performed using anti-DDX21 polyclonal antibody at 1μg/ml on various samples.
Lane 1: 293T cell lysate
Lane 2: K562 cell lysate

참고 문헌

데이터시트

Anti-Human DDX21 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-Human DDX21 Polyclonal Antibody (HP105014)

개요

이미지

참고 문헌

데이터시트

SDS

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