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Anti-Human DDX5 Polyclonal Antibody (HA418014)

Anti-Human DDX5 Polyclonal Antibody
Anti-Human DDX5 Polyclonal Antibody
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사양:
  • 50ug
  • 100ug
수량:
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  • 개요

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개요
카탈로그 번호HA418014
설명
Anti-Human DDX5 Polyclonal Antibody (HA418014) is a rabbit polyclonal antibody detecting DDX5. Suitable for Mouse and Human.
Highlights
  • ●Multi-Species — Cross-reactive for translational research.
종 반응성Human, Mouse
응용 분야ELISA, IHC, WB
숙주 종Rabbit
클론성Polyclonal
이소타입IgG
면역원 E. coli - derived recombinant Human DDX5 (Arg68-Ser483).
타겟 DDX5, DEAD box protein 5, EC:3.6.4.13, G17P1, HELR, HLR1, Probable ATP-dependent RNA helicase DDX5, RNA helicase p68
내독소 수준 Please contact with the lab for this information.
정제 Purified by antigen affinity column.
등록번호 P17844
형태 Liquid
저장 완충액 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보
응용 분야 희석
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
배경

Probable ATP-dependent RNA helicase DDX5 is a ~69 kDa protein. Involved in the alternative regulation of pre-mRNA splicing; its RNA helicase activity is necessary for increasing tau exon 10 inclusion and occurs in a RBM4-dependent manner. Binds to the tau pre-mRNA in the stem-loop region downstream of exon 10. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. Involved in transcriptional regulation; the function is independent of the RNA helicase activity. Transcriptional coactivator for androgen receptor AR but probably not ESR1.

참고사항 For research use only
이미지
  • Anti-Human DDX5 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-DDX5 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: NIH3T3 cell lysate
    Lane 2: 4T1 cell lysate
    Lane 3: RAW264.7 cell lysate
    Lane 4: Mouse brain lysate
    Lane 5: Rat brain lysate

참고 문헌
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
추천

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