Anti-Human DNM1 Polyclonal Antibody (HC395014)

Name: Anti-Human DNM1 Polyclonal Antibody
Brand: abinScience
SKU: HC395014
Price: 168 USD
Availability: InStock

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개요
이미지
참고 문헌
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SDS

개요

카탈로그 번호

HC395014

설명

Anti-Human DNM1 Polyclonal Antibody (HC395014) is a rabbit polyclonal antibody detecting DNM1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

종 반응성

Human, Mouse, Rat

응용 분야

ELISA, IHC, WB

숙주 종

Rabbit

클론성

Polyclonal

이소타입

IgG

면역원

E. coli - derived recombinant Human DNM1 (Met1-Arg271).

타겟

DNM1, DNM, Dynamin-1

정제

Purified by antigen affinity column.

등록번호

Q05193

형태

Liquid

저장 완충액

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보

응용 분야	희석
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

안정성 및 저장

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

배경

Dynamin-1 (DNM1) is a ~97 kDa protein. Catalyzes the hydrolysis of GTP and utilizes this energy to mediate vesicle scission and participates in many forms of endocytosis, such as clathrin-mediated endocytosis or synaptic vesicle endocytosis as well as rapid endocytosis (RE). Associates to the membrane, through lipid binding, and self-assembles into rings and stacks of interconnected rings through oligomerization to form a helical polymer around the vesicle membrane leading to constriction of invaginated coated pits around their necks. Self-assembly of the helical polymer induces membrane tubules narrowing until the polymer reaches a length sufficient to trigger GTP hydrolysis. Depending on the curvature imposed on the tubules, membrane detachment from the helical polymer upon GTP hydrolysis can cause spontaneous hemifission followed by complete fission. May play a role in regulating early stages of clathrin-mediated endocytosis in non-neuronal cells through its activation by dephosphorylation via the signaling downstream of EGFR.

1. Soulet, F. et al. (2005) Molecular biology of the cell 16, 2058-67. PMID: 15703209

2. Chappie, JS. et al. (2010) Nature 465, 435-40. PMID: 20428113

3. Srinivasan, S. et al. (2018) PLoS biology 16, e2005377. PMID: 29668686

4. van der Bliek, AM. et al. (1993) The Journal of cell biology 122, 553-63. PMID: 8101525

5. Lin, HC. et al. (1996) The Journal of biological chemistry 271, 27979-82. PMID: 8910402

6. Muhlberg, AB. et al. (1997) The EMBO journal 16, 6676-83. PMID: 9362482

7. Kong, L. et al. (2018) Nature 560, 258-262. PMID: 30069048

8. Hinshaw, JE. et al. (1995) Nature 374, 190-2. PMID: 7877694

9. Scaife, R. et al. (1998) Biochemistry 37, 17673-9. PMID: 9922133

10. Bashkirov, PV. et al. (2008) Cell 135, 1276-86. PMID: 19084269

참고사항

For research use only.

이미지

Western blot

Western blot analysis was performed using anti-DNM1 polyclonal antibody at 1μg/ml on various samples.
Lane 1: Mouse cerebellum lysate
Lane 2: Rat brain lysate
Lane 3: Rat cerebellum lysate

참고 문헌

데이터시트

Anti-Human DNM1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

재조합 단백질

항체

어세이 키트

Anti-Human DNM1 Polyclonal Antibody (HC395014)

개요

이미지

참고 문헌

데이터시트

SDS

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