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Anti-Human EHD1 Polyclonal Antibody (HP095014)

Anti-Human EHD1 Polyclonal Antibody
Anti-Human EHD1 Polyclonal Antibody
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  • 개요

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개요
카탈로그 번호HP095014
설명
Anti-Human EHD1 Polyclonal Antibody (HP095014) is a rabbit polyclonal antibody detecting EHD1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
종 반응성Human, Mouse, Rat
응용 분야ELISA, IHC, WB
숙주 종Rabbit
클론성Polyclonal
이소타입IgG
면역원 E. coli - derived recombinant Human EHD1 (Thr20-Ser402).
타겟 PAST, EHD1, PAST1, PAST homolog 1, EH domain-containing protein 1, Testilin, hPAST1
정제 Purified by antigen affinity column.
등록번호 Q9H4M9
형태 Liquid
저장 완충액 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요.

제품 사용 정보
응용 분야 희석
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
안정성 및 저장 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
배경

EH domain-containing protein 1 (EHD1) is a ~60 kDa protein. ATP- and membrane-binding protein that controls membrane reorganization/tubulation upon ATP hydrolysis. In vitro causes vesiculation of endocytic membranes. Acts in early endocytic membrane fusion and membrane trafficking of recycling endosomes. Recruited to endosomal membranes upon nerve growth factor stimulation, indirectly regulates neurite outgrowth. Plays a role in myoblast fusion.

1. Cai, B. et al. (2013) The Journal of biological chemistry 288, 30172-30180. PMID: 24019528
2. Naslavsky, N. et al. (2004) Molecular biology of the cell 15, 2410-22. PMID: 15020713
3. George, M. et al. (2007) BMC cell biology 8, 3. PMID: 17233914
4. Rahajeng, J. et al. (2010) The Journal of biological chemistry 285, 31918-22. PMID: 20801876
5. Xie, S. et al. (2019) Journal of cell science 132. PMID: 31615969
6. Lu, Q. et al. (2015) Nature cell biology 17, 228-240. PMID: 25686250
참고사항 For research use only.
이미지
  • Anti-Human EHD1 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-EHD1 polyclonal antibody at 1μg/ml on various samples.
    Lane 1: Mouse lung lysate
    Lane 2: Rat lung lysate

참고 문헌
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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