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| 카탈로그 번호 | HX179014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 설명 |
Anti-Human EIF4A1 Polyclonal Antibody (HX179014) is a rabbit polyclonal antibody detecting EIF4A1 in ELISA, IHC, WB. Suitable for Human.
Highlights
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| 종 반응성 | Human, Mouse, Rat | ||||||||
| 응용 분야 | ELISA, IHC, WB | ||||||||
| 숙주 종 | Rabbit | ||||||||
| 클론성 | Polyclonal | ||||||||
| 이소타입 | IgG | ||||||||
| 면역원 | E. coli - derived recombinant Human EIF4A1 (Thr242-Ile406). | ||||||||
| 타겟 | Eukaryotic initiation factor 4A-I, eIF4A-I, eIF-4A-I, EIF4A, EIF4A1, DDX2A, ATP-dependent RNA helicase eIF4A-1, | ||||||||
| 정제 | Purified by antigen affinity column. | ||||||||
| 등록번호 | P60842 | ||||||||
| 형태 | Liquid | ||||||||
| 저장 완충액 | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
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| 제품 사용 정보 |
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| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 배경 | Eukaryotic initiation factor 4A-I (EIF4A1) is a ~46 kDa protein. ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. As a result, promotes cell proliferation and growth. 1. Wolf, A. et al. (2010) The Journal of cell biology 188, 463-71. PMID: 20156963 | ||||||||
| 참고사항 | For research use only. |
Western blot analysis was performed using anti-EIF4A1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: NIH3T3 cell lysate
Lane 2: PC12 cell lysate
Lane 3: A431 cell lysate
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