中文
English
한국어
日本語
Español
Français
Русский
| 카탈로그 번호 | HY024014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 설명 |
Anti-Human EIF4E Polyclonal Antibody (HY024014) is a rabbit polyclonal antibody detecting EIF4E in ELISA, IHC, WB. Suitable for Human, Mouse, Rat.
Highlights
|
||||||||
| 종 반응성 | Human, Mouse, Rat | ||||||||
| 응용 분야 | ELISA, IHC, WB | ||||||||
| 숙주 종 | Rabbit | ||||||||
| 클론성 | Polyclonal | ||||||||
| 이소타입 | IgG | ||||||||
| 면역원 | E. coli - derived recombinant Human EIF4E (Asn30-Val217). | ||||||||
| 타겟 | Eukaryotic translation initiation factor 4E, EIF4F, mRNA cap-binding protein, eIF4E, eIF-4E, eIF-4F 25 kDa subunit, EIF4EL1, EIF4E | ||||||||
| 정제 | Purified by antigen affinity column. | ||||||||
| 등록번호 | P06730 | ||||||||
| 형태 | Liquid | ||||||||
| 저장 완충액 | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. 데이터시트 인쇄본 또는 로트별 COA에 기재된 구체적인 완충액 정보를 참조하세요. |
||||||||
| 제품 사용 정보 |
| ||||||||
| 안정성 및 저장 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 배경 | Eukaryotic translation initiation factor 4E (EIF4E) is a ~25 kDa protein. Acts in the cytoplasm to initiate and regulate protein synthesis and is required in the nucleus for export of a subset of mRNAs from the nucleus to the cytoplasm which promotes processes such as RNA capping, processing and splicing. Component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome. This protein recognizes and binds the 7-methylguanosine (m7G)-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Together with EIF4G1, antagonizes the scanning promoted by EIF1-EIF4G1 and is required for TISU translation, a process where the TISU element recognition makes scanning unnecessary. In addition to its role in translation initiation, also acts as a regulator of translation and stability in the cytoplasm. 1. McKendrick, L. et al. (2001) European journal of biochemistry 268, 5375-85. PMID: 11606200 2. Yanagiya, A. et al. (2012) Molecular cell 46, 847-58. PMID: 22578813 3. Chen, CC. et al. (2012) FEBS letters 586, 2260-6. PMID: 22684010 4. Kamenska, A. et al. (2014) Nucleic acids research 42, 3298-313. PMID: 24335285 5. Haimov, O. et al. (2018) Molecular and cellular biology 38. PMID: 29987188 6. Tomoo, K. et al. (2005) Biochimica et biophysica acta 1753, 191-208. PMID: 16271312 | ||||||||
| 참고사항 | For research use only. |
문의하기
+86-027-65523339
중국 우한시 심둔사로 666번지 C동, 우한, 430206
